User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/16: Difference between revisions
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*Based on the relatively static graph, or due to the lack in a shift in the spectra overtime shows that the HRP linked to the AuNP's is inactive. | *Based on the relatively static graph, or due to the lack in a shift in the spectra overtime shows that the HRP linked to the AuNP's is inactive. | ||
*This inactivity is most likely explained by the method of synthesis. If the heat was too high while preparing the HRP-linked nanoparticles it could have denatured the protein. | *This inactivity is most likely explained by the method of synthesis. If the heat was too high while preparing the HRP-linked nanoparticles it could have denatured the protein. | ||
[[Image:Oct_17_Abs_vs._time_@420nm.png]] | |||
*This graph represents the change in absorbance overtime at the wavelength where HRP is most active. Due to the fact that the graph in horizontal for all but the first point dictates that HRP most likely is inactive or its activity is interrupted by being linked to AuNP's. | |||
*The first point shows the solution at time zero before the HRP-AuNP's were added. As a result this big jump was to be expected. | |||
===UV-Vis Spectra of AU-NP's Currently Synthesized=== | ===UV-Vis Spectra of AU-NP's Currently Synthesized=== |
Revision as of 08:28, 22 October 2013
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Objective
ProcedureKinetics of HRP-AuNP'sSolutions Made
BSA Nanoparticle SynthesisProcedure for Synthesis can be found HERE.
UV-Vis Runs
DataHRP AU-NP's
UV-Vis Spectra of AU-NP's Currently Synthesized
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