User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/22: Difference between revisions

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* Stock Solutions: were as follows, Gold 2.52mM, HRP 15.3uM, and Pepsin 15.7 uM.  
* Stock Solutions: were as follows, Gold 2.52mM, HRP 15.3uM, and Pepsin 15.7 uM.  
[[Image:Oct_22_ratios.png]]
[[Image:Oct_22_ratios.png]]
*Once prepared, the test tubes were covered with foil and were baked for 4hrs at 80 degrees Celsius.


==Data==
==Data==

Revision as of 10:40, 22 October 2013

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Objective

  • Synthesize Pepsin linked AuNP's as shown by Dr.Hartings HERE.
  • Calculate the concentration ratio for Pepsin/AuNP and HRP-AuNP fiber formation.

Procedure

Ratios were made as follows

  • These ratios allow a constant concentration of Gold at .25mM, and corresponding protein concentration.
  • The amounts were measured using micropipettes.
  • Stock Solutions: were as follows, Gold 2.52mM, HRP 15.3uM, and Pepsin 15.7 uM.

  • Once prepared, the test tubes were covered with foil and were baked for 4hrs at 80 degrees Celsius.

Data


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