User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/22: Difference between revisions

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==Objective==
==Objective==
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==Data==
==Data==
 
*No data was taken today!!
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Revision as of 17:42, 29 October 2013

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • Synthesize Pepsin linked AuNP's as shown by Dr.Hartings HERE.
  • Calculate the concentration ratio for Pepsin/AuNP and HRP-AuNP fiber formation.

Procedure

Ratios were made as follows

  • These ratios allow a constant concentration of Gold at .25mM, and corresponding protein concentration.
  • The amounts were measured using micropipettes.
  • Stock Solutions: were as follows, Gold 2.52mM, HRP 15.3uM, and Pepsin 15.7 uM.

  • Once prepared, the test tubes were covered with foil and were baked for 4hrs at 80 degrees Celsius.

Data

  • No data was taken today!!


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