User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/23: Difference between revisions
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===UV-Vis of HRP and Pepsin AuNP's=== | ===UV-Vis of HRP and Pepsin AuNP's=== | ||
*In this section 1 ml of each HRP and Pepsin Linked AuNP sample was taken and centrifuged for 30 minutes. | |||
*Next the supernatant was removed to be run using UV-Vis. The pellet was saved for each sample. | |||
==Data== | ==Data== | ||
===UV-Vis Spectra=== | |||
*The graph below depicts the UV-Vis Spectra of all of the Pepsin linked AuNP samples. | |||
*Please note that all of the pepsin samples formed fibers, and were visually similar before they were run. | |||
[[Image:Oct_23_Pepsin_UVVis.png]] | [[Image:Oct_23_Pepsin_UVVis.png]] | ||
*The graph below depicts the UV-Vis Spectra of all of the HRP linked AuNP samples. | |||
*Please note none of the samples formed fibers; however, their was a visual decline in color from a deep red to purple color between the samples. | |||
[[Image:Oct_23_HRP_UVVIS.png]] | [[Image:Oct_23_HRP_UVVIS.png]] | ||
*Not all of the HRP Linked samples were run today because we ran out of time, as a result, only a few samples are shown above. | |||
===Sample Pictures=== | |||
[[Image:Oct_23_Pepsin_AuNP_set_1.png]] | |||
[[Image:Oct_23_Pepsin_AuNP_set_2.png]] | |||
[[Image:Oct_23_HRP_AuNP_set_1.png]] | |||
[[Image:Oct_23_HRP_AuNP_set_2.png]] | |||
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Revision as of 12:30, 28 October 2013
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Objective
ProcedureMaking HRP and Myoglobin AuNP's
UV-Vis of HRP and Pepsin AuNP's
DataUV-Vis Spectra
Sample Pictures |