User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/11/05: Difference between revisions

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*Using the procedure link above 5 ml samples of each of the specified nanoparticles samples were prepped for analysis.  
*Using the procedure link above 5 ml samples of each of the specified nanoparticles samples were prepped for analysis.  
*In relation to the gold standards a 10, 20, 30, 40 and 50 ug/ml were prepped for comparing to the AuNP's.
*In relation to the gold standards a 10, 20, 30, 40 and 50 ug/ml were prepped for comparing to the AuNP's.
*UV-Vis samples listed were run as they always have been. 1ml of sample was analyzed after centrifugation. We ensured that each sample had an absorbance less than 1 in order to analyze the data.


==Data==
==Data==

Revision as of 12:57, 11 November 2013

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Objective

  • Run Atomic Absorption Spectra of Myoglobin AuNP's (Both Sets), Lysozyme AuNP's made on Oct 30, and the HRP AuNP made on Oct 23 following Dr.Hartings procedure found HERE.

Procedure

  • Using the procedure link above 5 ml samples of each of the specified nanoparticles samples were prepped for analysis.
  • In relation to the gold standards a 10, 20, 30, 40 and 50 ug/ml were prepped for comparing to the AuNP's.
  • UV-Vis samples listed were run as they always have been. 1ml of sample was analyzed after centrifugation. We ensured that each sample had an absorbance less than 1 in order to analyze the data.

Data

UV-Vis on Lysozyme and Myoglobin Nanoparticles made on Oct 30, 2013

Atomic Absorption Spectra of Myoglobin AuNP's (Both Sets), Lysozyme AuNP's made on Oct 30, and the HRP AuNP made on Oct 23

  • The graph below represents the gold standards, [] vs. absorbance.

  • The graphs below represent the AA spectra's of the samples listed above showing synthesized ratio vs. [].


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