User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/01/29: Difference between revisions

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==Procedure==
==Procedure==
===Chelators===
===Chelators===
# Shake each test tube (40, 50, 110 ratio) of lysozyme-AuNP until fibers appear to be evenly distributed throughout.
* Shake each test tube (40, 50, 110 ratio) of lysozyme-AuNP until fibers appear to be evenly distributed throughout.
# Take 1 mL aliquots from each test tube and place in 1.5 mL tubes.  
* Take 1 mL aliquots from each test tube and place in 1.5 mL tubes.  


'''Creating Stock Solutions of Chelators'''
====Creating Stock Solutions of Chelators====


[[Image:1.29.data.chelator.stock]]


'''Adding Chelators to Lysozyme-AuNP Solutions'''
# Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.
# Record effects of each chelator on each solution.


[[Image:1.29.data.chelator.add]]
====Adding Chelators to Lysozyme-AuNP Solutions====
* Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.  
* Record effects of each chelator on each solution.


===pH/Buffers===
===pH/Buffers===

Revision as of 11:33, 1 February 2014

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Purpose

Procedure

Chelators

  • Shake each test tube (40, 50, 110 ratio) of lysozyme-AuNP until fibers appear to be evenly distributed throughout.
  • Take 1 mL aliquots from each test tube and place in 1.5 mL tubes.

Creating Stock Solutions of Chelators

Adding Chelators to Lysozyme-AuNP Solutions

  • Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.
  • Record effects of each chelator on each solution.

pH/Buffers

Data


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