User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/01/29: Difference between revisions

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===pH/Buffers===
===pH/Buffers===
====Adding Buffers to Lysozyme-AuNP Solutions====
* Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM.
* Add 1 mL of each to the lysozyme-AuNP solutions.
* Let sit overnight and record results.


==Data==
==Data==

Revision as of 11:35, 1 February 2014

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Purpose

Procedure

Chelators

  • Shake each test tube (40, 50, 110 ratio) of lysozyme-AuNP until fibers appear to be evenly distributed throughout.
  • Take 1 mL aliquots from each test tube and place in 1.5 mL tubes.

Creating Stock Solutions of Chelators

Adding Chelators to Lysozyme-AuNP Solutions

  • Add 0.5 mL of each chelator stock solution to each 1 mL aliquot.
  • Record effects of each chelator on each solution.

pH/Buffers

Adding Buffers to Lysozyme-AuNP Solutions

  • Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM.
  • Add 1 mL of each to the lysozyme-AuNP solutions.
  • Let sit overnight and record results.

Data


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