User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/01/29: Difference between revisions
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* Let sit overnight and record results. | * Let sit overnight and record results. | ||
===Looking at the AuNP's=== | |||
*None of the 50:1 ratio AuNP's formed fibers. As a result they were remade because one of the stocks must have been slightly less concentrated. | |||
*Carly re-did the calculations and remade more 50:1 AuNP's. Nonetheless the chelators and and buffers were added to the other ratios made to see the effect. We will see the effect on the 50:1 ratio tomorrow. | |||
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Revision as of 08:56, 5 February 2014
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Purpose
Procedure
ChelatorsCreating Stock Solutions of Chelators
Adding Chelators to Lysozyme-AuNP Solutions
pHHow we made each 1 M Buffer Solution
Citric Acid: pH - 3
Phosphate: pH - 7
Tris: pH - 9
MES: pH - 5.21 (Note Cannot Get Down to 5)
HEPES: pH - 7
CHES: pH - 9
Adding Buffers to Lysozyme-AuNP Solutions
Looking at the AuNP's
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