User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/04: Difference between revisions

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'''Adding Chelators to Lysozyme-AuNP Solutions'''
'''Adding Chelators to Lysozyme-AuNP Solutions'''
# Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.  
* Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.  
# Record effects of each chelator on each solution.  
* Record effects of each chelator on each solution.  


*Chelators are basic because NaOH had to be added to dissolve the respective chelators with the exception of 2,2 bipyridine.
*Chelators are basic because NaOH had to be added to dissolve the respective chelators with the exception of 2,2 bipyridine.
[[Image:1.29.data.chelator.add|800px|]]
[[Image:1.29.data.chelator.add|800px|]]
* 2,2 bipyridine was used instead of 2,2 bipyridyl
* 2,2 bipyridine was used instead of 2,2 bipyridyl
====Note for all Chelators with the exception of 2,2 bipyridine, NaOH had to be added in order to get the chelator to dissolve in water. Each pH of the chelator were around a pH of 9-10. This was tested with pH strips.====


===pH===
===pH===

Revision as of 18:52, 8 February 2014

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Purpose/Objective

  • Synthesize lysozyme-AuNP.
  • Create stock solutions of chelators.
  • Add the various chelators to lysozyme-AuNP solutions.
  • Change pH of lysozyme-AuNP solutions.
  • Record results.

Procedure

Chelators

Creating Stock Solutions of Chelators

  • 2,2 Bipyridine was used instead of 2, 2 bipyridyl. It stillhas the same function just a different chirality of the 2 nitrogen's.

Adding Chelators to Lysozyme-AuNP Solutions

  • Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot.
  • Record effects of each chelator on each solution.
  • Chelators are basic because NaOH had to be added to dissolve the respective chelators with the exception of 2,2 bipyridine.

  • 2,2 bipyridine was used instead of 2,2 bipyridyl

Note for all Chelators with the exception of 2,2 bipyridine, NaOH had to be added in order to get the chelator to dissolve in water. Each pH of the chelator were around a pH of 9-10. This was tested with pH strips.

pH

Adding Buffers to Lysozyme-AuNP Solutions

  1. Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM.
  2. Add 1 mL of each to the lysozyme-AuNP solutions.
  3. Let sit overnight and record results.

Made More AuNP

  • Because the 50:1 ratio did not form fibers as expected during our last round of synthesis we decided to make more AuNP's.
  • Specifically we made more 40:1 and 50:1 lysozyme lined AuNP's.
  • ASK CARLY FOR CALCULATIONS!!


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