User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Data==
==Objective==
*The 55:1 and 110:1 lysozyme "spots" were just pure fibers with variable added. The 100:1 BSA samples had no fibers and then we added the variable.  
* To synthesize lysozyme-AuNPs.
* Finish tests on 55:1 and 110:1 lysozyme-AuNPs as well as 100:1 BSA-AuNPs.
* Image test results from last week.
 
 
==Procedure==
'''Creating Stock Solutions of Gold and Lysozyme'''
* As seen below, each stock solution was created following the calculations in the table.
* Each solution was created using a volumetric flask and deionized water.
[[Image:2.18.data.png|800px|]]
 
 
'''Synthesizing Lysozyme-AuNPs'''
* As seen below, each solutions was created using the following calculations.
* We made ten tubes of each ratio.
[[Image:cheatsheet.png|800px|]]
 
==Images==
*The 55:1 and 110:1 lysozyme samples consisted of pure fibers with variable added. However, the 100:1 BSA samples had no fibers before adding the variables.
[[Image:Feb_18_Pic_3.jpg|300px]]<br.>
[[Image:Feb_18_Pic_3.jpg|300px]]<br.>


Revision as of 15:29, 24 February 2014

Biomaterials Design Lab: Spring 2014 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • To synthesize lysozyme-AuNPs.
  • Finish tests on 55:1 and 110:1 lysozyme-AuNPs as well as 100:1 BSA-AuNPs.
  • Image test results from last week.


Procedure

Creating Stock Solutions of Gold and Lysozyme

  • As seen below, each stock solution was created following the calculations in the table.
  • Each solution was created using a volumetric flask and deionized water.


Synthesizing Lysozyme-AuNPs

  • As seen below, each solutions was created using the following calculations.
  • We made ten tubes of each ratio.

Images

  • The 55:1 and 110:1 lysozyme samples consisted of pure fibers with variable added. However, the 100:1 BSA samples had no fibers before adding the variables.

<br.>

<br.>

<br.>

<br.>

<br.>



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab/2014/02/18&oldid=776567"