User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/18

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{|{{table}} width="800"
{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Data==
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==Objective==
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*The 55:1 and 110:1 lysozyme "spots" were just pure fibers with variable added. The 100:1 BSA samples had no fibers and then we added the variable.  
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* To synthesize lysozyme-AuNPs.
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* Finish tests on 55:1 and 110:1 lysozyme-AuNPs as well as 100:1 BSA-AuNPs.
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* Image test results from last week.
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==Procedure==
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'''Creating Stock Solutions of Gold and Lysozyme'''
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* As seen below, each stock solution was created following the calculations in the table.
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* Each solution was created using a volumetric flask and deionized water.
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[[Image:2.18.data.png|800px|]]
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'''Synthesizing Lysozyme-AuNPs'''
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* As seen below, each solutions was created using the following calculations.
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* We made ten tubes of each ratio.
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[[Image:cheatsheet.png|800px|]]
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==Images==
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*The 55:1 and 110:1 lysozyme samples consisted of pure fibers with variable added. However, the 100:1 BSA samples had no fibers before adding the variables.
[[Image:Feb_18_Pic_3.jpg|300px]]<br.>
[[Image:Feb_18_Pic_3.jpg|300px]]<br.>

Revision as of 18:29, 24 February 2014

Biomaterials Design Lab: Spring 2014 Main project page
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Objective

  • To synthesize lysozyme-AuNPs.
  • Finish tests on 55:1 and 110:1 lysozyme-AuNPs as well as 100:1 BSA-AuNPs.
  • Image test results from last week.


Procedure

Creating Stock Solutions of Gold and Lysozyme

  • As seen below, each stock solution was created following the calculations in the table.
  • Each solution was created using a volumetric flask and deionized water.


Synthesizing Lysozyme-AuNPs

  • As seen below, each solutions was created using the following calculations.
  • We made ten tubes of each ratio.

Images

  • The 55:1 and 110:1 lysozyme samples consisted of pure fibers with variable added. However, the 100:1 BSA samples had no fibers before adding the variables.








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