User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/19: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Data==
==Objective==
*Salts all form fibers or aggregates that disperse in solution on transfer but settle overtime.  
* Synthesize lysozyme-AuNPs with salt added before being heated.
* Image tests from yesterday.
* Prepare diluted solutions of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl<sub>2</sub>, CaCl<sub>2</sub> from 1 M to 100 mM, 10 mM, and 1 mM.
* Experiment with various concentrations to determine the minimum concentration of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl<sub>2</sub>, CaCl<sub>2</sub>, HEPES, and Tris that causes fiber formation.
 
==Procedure==
'''Creating Stock Solutions of Gold and Lysozyme'''
* As seen below, each stock solution was created following the calculations in the table.
* Each solution was created using a volumetric flask and deionized water.
 
[[Image:stock.2.19.png|700px|]]
 
 
'''Synthesizing Lysozyme-AuNPs'''
* As seen below, each solutions was created using the following calculations.
* We made one tube of each ratio for the following 1 M salt solutions: KCl, NaCl, CaCl<sub>2</sub>, MgCl<sub>2</sub>
 
[[Image:2.19.png|800px|]]
 
 
'''Adding Diluted Variables to the Lysozyme-AuNP Samples'''
* Dilute the stock solutions of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl<sub>2</sub>, and CaCl<sub>2</sub> to 100 mM, 10 mM, and 1 mM.
* Add 0.5 mL of each variable to the 30, 55, and 110 ratios of lysozyme-AuNPs.
* Let sit on the bench overnight.
 
==Images==
*Salts all form fibers or aggregates that disperse in solution but settle overtime.  
 
[[Image:Feb_19,_2014_Pic_1.jpg|300px]]<br.>
[[Image:Feb_19,_2014_Pic_1.jpg|300px]]<br.>


[[Image:Feb_19,_2014_Pic_2.jpg|300px]]
[[Image:Feb_19,_2014_Pic_2.jpg|300px]]


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Revision as of 15:31, 24 February 2014

Biomaterials Design Lab: Spring 2014 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Synthesize lysozyme-AuNPs with salt added before being heated.
  • Image tests from yesterday.
  • Prepare diluted solutions of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl2, CaCl2 from 1 M to 100 mM, 10 mM, and 1 mM.
  • Experiment with various concentrations to determine the minimum concentration of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl2, CaCl2, HEPES, and Tris that causes fiber formation.

Procedure

Creating Stock Solutions of Gold and Lysozyme

  • As seen below, each stock solution was created following the calculations in the table.
  • Each solution was created using a volumetric flask and deionized water.


Synthesizing Lysozyme-AuNPs

  • As seen below, each solutions was created using the following calculations.
  • We made one tube of each ratio for the following 1 M salt solutions: KCl, NaCl, CaCl2, MgCl2


Adding Diluted Variables to the Lysozyme-AuNP Samples

  • Dilute the stock solutions of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl2, and CaCl2 to 100 mM, 10 mM, and 1 mM.
  • Add 0.5 mL of each variable to the 30, 55, and 110 ratios of lysozyme-AuNPs.
  • Let sit on the bench overnight.

Images

  • Salts all form fibers or aggregates that disperse in solution but settle overtime.

<br.>



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