User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/25: Difference between revisions
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==Objective== | |||
* Spin down samples of 30:1 lysozyme-AuNP with 1M salts added and run UV-Vis. | |||
* Heat samples 30:1 lysozyme-AuNP with 1M salts at 37°C overnight. | |||
* Image results of 30:1, 55:1, and 110:1 lysozyme-AuNPs synthesized with salt added before heating using UV-Vis. | |||
* Run atomic absorption of 30:1, 55:1, and 110:1 lysozyme-AuNPs synthesized with salts added before heating. | |||
==Procedure== | |||
===Atomic Absorption=== | |||
'''Creating the Gold Stock Solutions''' | |||
# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. | |||
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
'''Atomic Absorption Samples''' | |||
Solutions with the following Au:lysozyme ratio were run on the AA: | |||
* 30:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl | |||
* 55:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl | |||
* 110:1 MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl | |||
==Images== | |||
===Atomic Absorance of Lysozyme with KCl, NaCl, CaCl<sub>2</sub>, MgCl<sub>2</sub>=== | |||
''Figure 1. Gold:protein Ratio to Concentration of Lysozyme-AuNP Baked with Salts'' | |||
[[Image:Lysozyme Gold-protein to Concentration with Salts, JAC.png|550px|]] | |||
==UV-Vis== | ==UV-Vis== | ||
===UV-Vis For Solutions that Contained Salt Before Lysozyme AuNP Synthesis=== | ===UV-Vis For Solutions that Contained Salt Before Lysozyme AuNP Synthesis=== | ||
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===UV-Vis For Select Variables at More Refined Concentrations=== | ===UV-Vis For Select Variables at More Refined Concentrations=== | ||
*Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. | |||
[[Image:Screen_shot_2014-02-25_at_11.47.14_PM.png]] | [[Image:Screen_shot_2014-02-25_at_11.47.14_PM.png]] | ||
[[Image:Screen_shot_2014-02-25_at_11.46.46_PM.png]] | [[Image:Screen_shot_2014-02-25_at_11.46.46_PM.png]] | ||
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[[Image:Screen_shot_2014-02-25_at_11.44.28_PM.png]] | [[Image:Screen_shot_2014-02-25_at_11.44.28_PM.png]] | ||
[[Image:Screen_shot_2014-02-25_at_11.44.01_PM.png]] | [[Image:Screen_shot_2014-02-25_at_11.44.01_PM.png]] | ||
*Based off of all UV-Vis data: The ABS for the controls were corrected for the dilution factor present and the percent AuNP's in solution was calculated. | |||
[[Image:Screen_Shot_2014-03-26_at_2.49.43_PM.png]] | |||
==Notes== | |||
[[Image:notes.2.25.jac.png|700px|]] | |||
* These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio with salts added after. | |||
[[Image:more.2.25.notes.png|500px|]] | |||
* These results detail the reaction of the different ratios synthesized with salts added before heating. | |||
Revision as of 11:53, 26 March 2014
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Objective
ProcedureAtomic AbsorptionCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio were run on the AA:
ImagesAtomic Absorance of Lysozyme with KCl, NaCl, CaCl2, MgCl2Figure 1. Gold:protein Ratio to Concentration of Lysozyme-AuNP Baked with Salts UV-VisUV-Vis For Solutions that Contained Salt Before Lysozyme AuNP SynthesisUV-Vis For Select Variables at More Refined Concentrations
Notes
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