User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/26: Difference between revisions

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==UV-Vis Variable Heated Samples (36 Degrees Celcius)==
==Objective==
[[Image:Screen_shot_2014-02-26_at_3.32.15_PM.png]]
* Record results of 30:1 lysozyme:AuNP (with variables added after baking) that were heated at 37°C overnight.  
[[Image:Screen_shot_2014-02-26_at_3.32.02_PM.png]]
* Take UV-Vis of samples with salt added after baking heated at 37°C overnight.
[[Image:Screen_shot_2014-02-26_at_3.31.50_PM.png]]
* Synthesize 30:1 lysozyme-AuNP solutions.
[[Image:Screen_shot_2014-02-26_at_3.31.40_PM.png]]
* Synthesize 60:1, 70:1, 80:1, 90:1, and 100:1 BSA-AuNPs baked with MgCl<sub>2</sub> for 4 hours at 80°C.
[[Image:Screen_shot_2014-02-26_at_3.31.31_PM.png]]
[[Image:Screen_shot_2014-02-26_at_3.31.19_PM.png]]
[[Image:Screen_shot_2014-02-26_at_3.31.07_PM.png]]


==Procedure==
===Synthesizing Lysozyme-AuNP and BSA-AuNP===
* The following figure details how the stock solutions were made as well as the BSA-AuNPs and lysozyme-AuNPs.
* One tube of each ratio was made for BSA-AuNPs.
* For lysozyme-AuNPs, 25 tubes were made.
* The samples were heated in the oven at 80°C for 4 hours.


[[Image:lysbsa.2.26.png|700px|]]
==Figures==
*Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Also the Control Used is the Control at Room Temperature, not at 36 Degrees.
===UV-Vis of Heated Samples and Variables (36°C)===
[[Image:Screen_shot_2014-02-26_at_5.18.42_PM.png]]
[[Image:Screen_shot_2014-02-26_at_5.18.29_PM.png]]
[[Image:Screen_shot_2014-02-26_at_5.18.18_PM.png]]
[[Image:Screen_shot_2014-02-26_at_5.17.57_PM.png]]
[[Image:Screen_shot_2014-02-26_at_5.17.46_PM.png]]
[[Image:Screen_shot_2014-02-26_at_5.17.36_PM.png]]
[[Image:Screen_shot_2014-02-26_at_5.17.23_PM.png]]
*Based off of all UV-Vis data: The ABS for the controls were corrected for the dilution factor present and the percent AuNP's in solution was calculated.
[[Image:Screen_Shot_2014-03-26_at_2.53.43_PM.png]]
==Notes==
[[Image:afterheating.png|700px|]]
* These results detail the same samples as seen below; however, these samples were at 37°C before observing.
* After observations were taken, the samples were placed in the fridge.
[[Image:notes.2.25.jac.png|700px|]]
* These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio with salts added after. The samples were at room temperature (37°C) when observations were taken.
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Revision as of 11:55, 26 March 2014

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Objective

  • Record results of 30:1 lysozyme:AuNP (with variables added after baking) that were heated at 37°C overnight.
  • Take UV-Vis of samples with salt added after baking heated at 37°C overnight.
  • Synthesize 30:1 lysozyme-AuNP solutions.
  • Synthesize 60:1, 70:1, 80:1, 90:1, and 100:1 BSA-AuNPs baked with MgCl2 for 4 hours at 80°C.

Procedure

Synthesizing Lysozyme-AuNP and BSA-AuNP

  • The following figure details how the stock solutions were made as well as the BSA-AuNPs and lysozyme-AuNPs.
  • One tube of each ratio was made for BSA-AuNPs.
  • For lysozyme-AuNPs, 25 tubes were made.
  • The samples were heated in the oven at 80°C for 4 hours.

Figures

  • Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Also the Control Used is the Control at Room Temperature, not at 36 Degrees.

UV-Vis of Heated Samples and Variables (36°C)

  • Based off of all UV-Vis data: The ABS for the controls were corrected for the dilution factor present and the percent AuNP's in solution was calculated.

Notes

  • These results detail the same samples as seen below; however, these samples were at 37°C before observing.
  • After observations were taken, the samples were placed in the fridge.



  • These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio with salts added after. The samples were at room temperature (37°C) when observations were taken.


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