User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/04: Difference between revisions

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==Notes==
==Notes==

Revision as of 11:56, 26 March 2014

Biomaterials Design Lab: Spring 2014 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • Add the following variables to the 30:1 lysozyme-AuNPs synthesized without salts: NaCl, KCl, MgCl2, CaCl2, 2,2 bipyridine, MES, citric acid.
  • Run UV-Vis of 30:1 lysozyme:AuNP (with variables added after baking) that were cooled to 4°C over the weekend.

Procedure

Preparing Samples for Atomic Absorption

  • To the 30:1 lysozyme-AuNP samples synthesized with salts, 2.71 mL of the variables were added. Three concentrations for each were tested: 100 mM, 10 mM, and 1 mM.
  • For 2,2 bipyridine, the concentrations were 9 mM, 0.9 mM, and 0.09 mM.
  • The samples were left to sit at room temperature overnight.

Figures

  • Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Also the Control Used is the Control at Room Temperature, not at 4 Degrees.

Notes

Observations of 30:1 lysozyme-AuNP Samples Cooled to 4°C With Variables Added After Synthesizing


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