User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/05: Difference between revisions
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'''Creating the Gold Stock Solutions''' | '''Creating the Gold Stock Solutions''' | ||
* Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
* Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
* Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
* Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. | |||
* Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
* A sixth stock solution of gold was created with 448 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4552 μL of distilled water to a Falcon tube. This resulting concentration represents the amount of gold in a 5 mL sample of lysozyme-AuNP. | |||
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=== | ===Atomic Absorbance of BSA with MgCl2 added before being heated=== | ||
*With the BSA samples baked with salt their doesn't appear to be any recognizable trend. | *With the BSA samples baked with salt their doesn't appear to be any recognizable trend. | ||
[[Image:BSA with MgCL2 Javier Vinals Camallonga.png]] | [[Image:BSA with MgCL2 Javier Vinals Camallonga.png]] |
Revision as of 15:43, 17 April 2014
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Objective
ProcedureAtomic AbsorptionCreating the Gold Stock Solutions
Atomic Absorption Samples The following samples were run on the AA:
FiguresAtomic absorbance of Lysozyme with different concentrations of variables
Atomic Absorbance of BSA with MgCl2 added before being heated
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