User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/18

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(UV-Vis Lyso 30:1)
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*Note that dilution of the control was not necessary. We realized afterwards that the control used to make this graph was not diluted like the variable samples, as a result, the actual absorbance at the peak is closer to were the peak with variable is.
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Revision as of 18:38, 17 April 2014

Biomaterials Design Lab: Spring 2014 Main project page
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Objective

  • Run UV-Vis of all ratios of BSA-AuNP and 30:1 lysozyme-AuNP samples with 2,2 bipyridine.
  • Take conductivity of 60:1, 70:1, 80:1, 90:1, and 100:1 BSA-AuNPs baked with MgCl2 measured at room temperature.
  • Calculate equilibrium constants for heated 30:1 lysozyme:AuNP (with variables added after baking) at 37°C, room temperature 30:1 lysozyme:AuNP (with variables added after baking), and cooled 30:1 lysozyme:AuNP (with variables added after baking) at 4°C.

Images

Conductivity Readings of 30:1 Lysozyme-AuNP Samples with Variables Added After Baking

  • Note that water had to be added to the solutions to bring the volume up to 11ml. This was done because the sensor could take a measurement if we had a volume less than 12.

UV-Vis Lyso 30:1

Image:Screen_Shot_2014-03-18_at_3.00.24_PM.png Image:Screen_Shot_2014-03-18_at_3.00.38_PM.png Image:Screen_Shot_2014-03-18_at_3.00.48_PM.png Image:Screen_Shot_2014-03-18_at_3.01.00_PM.png Image:Screen_Shot_2014-03-18_at_3.01.11_PM.png Image:Screen_Shot_2014-03-18_at_3.01.57_PM.png Image:Screen_Shot_2014-03-19_at_3.14.08_PM.png

  • Note that dilution of the control was not necessary. We realized afterwards that the control used to make this graph was not diluted like the variable samples, as a result, the actual absorbance at the peak is closer to were the peak with variable is.


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