User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/25: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Objective==
==Objective==
* Add three concentrations (30, 60, 90 mM) of variables to the 30:1 lysozyme-AuNP solutions at three temperatures (4°C, 22°C, 36°C.
*More Lyso 30:1 AuNP's were synthesized in order to test new concentrations of variables. Concentrations 30mM, 60mM and 90mM were chosen because these ratios appear to fill in the gaps for the previous UV-Vis performed this semester in lab.
* Synthesize an additional tube of 30:1 lysozyme-AuNP to use as a standard for future testing.
*We plan to analyzes three sets of these AuNP at three temperatures again but also run AA and conductivity measurements at each temp. To achieve this three sets of AuNP's, one for each temp were synthesized for analysis at 4, 22, and 36 degrees Celsius.


==Procedure==
==Procedure==

Revision as of 20:48, 31 March 2014

Biomaterials Design Lab: Spring 2014 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • More Lyso 30:1 AuNP's were synthesized in order to test new concentrations of variables. Concentrations 30mM, 60mM and 90mM were chosen because these ratios appear to fill in the gaps for the previous UV-Vis performed this semester in lab.
  • We plan to analyzes three sets of these AuNP at three temperatures again but also run AA and conductivity measurements at each temp. To achieve this three sets of AuNP's, one for each temp were synthesized for analysis at 4, 22, and 36 degrees Celsius.

Procedure

Preparing Samples for Addition of Variables

  • Pipette 5 mL aliquots into a Falcon tube from the 15 mL samples.


Dilution of Variables

  • The following table shows how we diluted the stock concentrations of various variables.
  • The first graph shows the dilution to 30 mM, the second graph shows 60 mM, and the third graph shows 90 mM.
  • The concentrations of 2,2 bipyridine used are 0.0002316 M, 0.0004544 M, and 0.0006772 M from a stock concentration of 0.0099 M.


Adding Variables to 30:1 Lysozyme-AuNP Solutions

  1. Add 2.5 mL of each variable to each tube.
  2. Place 21 tubes in the oven at 36°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine.
  3. Place 21 tubes in the freezer at 4°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine.
  4. Place 21 tubes on the bench at 22°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine.


Synthesizing 30:1 Lysozyme-AuNP

  • The following figure describes how we made one tube of 30:1 lysozyme-AuNPs.
  • The sample was heated at 80°C for 4 hours.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab/2014/03/25&oldid=784583"