User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/26: Difference between revisions
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== | ==Objective== | ||
* | * Run UV-Vis and AA on room temperature samples from 3/25/14. | ||
* Synthesize more 30:1 lysozyme-AuNPs to repeat testing from yesterday. | |||
==Procedure== | |||
===Lysozyme-AuNPs=== | |||
'''Creating Stock Solutions of Gold and Lysozyme''' | |||
* As seen below, each stock solution was created following the calculations in the table. | |||
* Each solution was created using a volumetric flask and deionized water. | |||
[[Image:3.26.stock.png|650px|]] | |||
'''Synthesizing Lysozyme-AuNPs''' | |||
* As seen below, each solutions was created using the following calculations. | |||
* We made 66 tubes total. | |||
* Note: Instead of adding each amount into the tubes individually, we added the total volumes for 66 tubes into a beaker. We then measured 5 mL, which were transferred to glass test tubes. | |||
[[Image:3.26.data.png|650px|]] | |||
===Atomic Absorption=== | |||
'''Creating the Gold Stock Solutions''' | |||
# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. | |||
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
'''Atomic Absorption Samples''' | |||
Solutions with the following Au:lysozyme ratio were run on the AA: | |||
* 30:1 lysozyme-AuNP with 0.03 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) | |||
* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | |||
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | |||
==Figures== | |||
[[Image:3.26.aa.png|750px|]] | |||
Revision as of 20:29, 31 March 2014
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Objective
ProcedureLysozyme-AuNPsCreating Stock Solutions of Gold and Lysozyme
Atomic AbsorptionCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio were run on the AA:
Figures
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