User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/03/26: Difference between revisions

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==UV-Vis Graphs==
==UV-Vis Graphs==
*No 30:1 Lyso AuNP Control was Run because no samples were leftover from this batch of AuNP's. A single tube was made, but a mistake was made in its preparation causing fibers to form.
[[Image:Screen_Shot_2014-03-31_at_11.28.08_PM.png]]
[[Image:Screen_Shot_2014-03-31_at_11.28.08_PM.png]]
[[Image:Screen_Shot_2014-03-31_at_11.28.28_PM.png]]
[[Image:Screen_Shot_2014-03-31_at_11.28.28_PM.png]]

Revision as of 20:34, 31 March 2014

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Objective

  • Run UV-Vis and AA on room temperature lysozyme AuNP samples from 3/25/14.
  • Synthesize more 30:1 lysozyme-AuNPs to repeat testing from yesterday.(This was done because the UV-Vis data run for point number 1 was wonky).

Procedure

Lysozyme-AuNPs

Creating Stock Solutions of Gold and Lysozyme

  • As seen below, each stock solution was created following the calculations in the table.
  • Each solution was created using a volumetric flask and deionized water.


Synthesizing Lysozyme-AuNPs

  • As seen below, each solutions was created using the following calculations.
  • We made 66 tubes total.
  • Note: Instead of adding each amount into the tubes individually, we added the total volumes for 66 tubes into a beaker. We then measured 5 mL, which were transferred to glass test tubes.

Atomic Absorption

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

AA Graphs

UV-Vis Graphs

  • No 30:1 Lyso AuNP Control was Run because no samples were leftover from this batch of AuNP's. A single tube was made, but a mistake was made in its preparation causing fibers to form.


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