User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/01: Difference between revisions

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===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature===
===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature===
*Water was added to sample in order to increase the volume above 11ml. This was done because 11ml of solution was needed in order for the detector to measure the conductivity of the sample.
[[Image:4.1.conductivity.jac.png|800px|]]
[[Image:4.1.conductivity.jac.png|800px|]]
*PLEASE NOE THAT THESE CONDUCTIVITY MEASUREMENTS ARE NOT CORRECTED FOR THE DILUTION FACTOR.
*PLEASE NOTE THAT THESE CONDUCTIVITY MEASUREMENTS ARE NOT CORRECTED FOR THE DILUTION FACTOR.


===Atomic Absorption Preparation===
===Atomic Absorption Preparation===


'''Creating the Gold Stock Solutions'''
'''Creating the Gold Stock Solutions'''
# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
* Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
* Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
* Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
* Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
* Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.




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* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)


===Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples===
===Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples===
# 2.841 g of sodium citrate was added to a 15 ml falcon tube and was filled with deionized water to 10 mL mark.  
* 2.841 g of sodium citrate was added to a 15 ml falcon tube and was filled with deionized water to 10 mL mark.  
# Dilutions for 1 mM, 10 mM, and 100 mM solutions were prepped.
* Dilutions for 1 mM, 10 mM, and 100 mM solutions were prepped.
# 1 mL aliquots were taken from 30:1 lysozyme-AuNP samples and were added 0.5 mL of each concentration of sodium citrate.
* 1 mL aliquots were taken from 30:1 lysozyme-AuNP samples and were added 0.5 mL of each concentration of sodium citrate.


==Figures==
==Figures==

Revision as of 15:16, 17 April 2014

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Objective

  • We plan to run AA and UV-Vis on 30:1 lysozyme linked gold nanoparticle samples from 3/25/14 (Only the 4 Degree Celsius samples were run).
  • We also plan to run conductivity measurements on room temperature 30:1 lysozyme linked gold nanoparticle samples from 3/25/14.

Procedure

Adding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/14

1. Add 2.25 mL of each variable to 4.5 mL of sample.

  • The variables used were:
  • 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.

Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature

  • Water was added to sample in order to increase the volume above 11ml. This was done because 11ml of solution was needed in order for the detector to measure the conductivity of the sample.

  • PLEASE NOTE THAT THESE CONDUCTIVITY MEASUREMENTS ARE NOT CORRECTED FOR THE DILUTION FACTOR.

Atomic Absorption Preparation

Creating the Gold Stock Solutions

  • Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  • Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  • Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  • Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  • Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples

  • 2.841 g of sodium citrate was added to a 15 ml falcon tube and was filled with deionized water to 10 mL mark.
  • Dilutions for 1 mM, 10 mM, and 100 mM solutions were prepped.
  • 1 mL aliquots were taken from 30:1 lysozyme-AuNP samples and were added 0.5 mL of each concentration of sodium citrate.

Figures

  • THIS DATA IS NO LONGER OF USE TO US. THIS IS BECAUSE THESE SAMPLES WERE PREPARED IN PLASTIC. THE RESULTS SEEN IN SAMPLE DON'T FOLLOW ANY OF THE TRENDS OBSERVED WHEN SAMPLES WERE MADE IN GLASS. WE DECIDED TO DISREGARD THESE RESULTS IN FAVOR OF LOOKING AT THE SAMPLES MADE IN GLASS.


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