User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/01: Difference between revisions
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'''Creating the Gold Stock Solutions''' | '''Creating the Gold Stock Solutions''' | ||
* Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. | |||
* Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. | |||
* Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. | |||
* Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. | |||
* Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. | |||
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* 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | * 30:1 lysozyme-AuNP with 0.06 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) | ||
* 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | * 30:1 lysozyme-AuNP with 0.09 M MgCl<sub>2</sub>, CaCl<sub>2</sub>, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) | ||
===Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples=== | ===Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples=== |
Revision as of 15:15, 17 April 2014
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Objective
ProcedureAdding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/141. Add 2.25 mL of each variable to 4.5 mL of sample.
2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C. Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature
Atomic Absorption PreparationCreating the Gold Stock Solutions
Atomic Absorption Samples Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:
Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples
Figures
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