User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/08

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(Conductivity Measurement of Pure Variables at Room Temperature)
(Atomic Absorption Preparation)
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'''Creating the Gold Stock Solutions'''
'''Creating the Gold Stock Solutions'''
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# Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
+
* Add 50 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
-
# Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
+
* Add 100 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
-
# Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
+
* Add 150 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
-
# Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
+
* Add 200 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
-
# Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
+
* Add 250 μL of HAuCl<sub>4</sub>·3H<sub>2</sub>O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.

Revision as of 17:12, 17 April 2014

Biomaterials Design Lab: Spring 2014 Main project page
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Objective

  • Take conductivity measurements on room temperature solutions from 3/26/14.
  • Run UV-Vis and AA on solutions at 4°C from 3/26/14.

Procedure

Conductivity Measurement of Pure Variables at Room Temperature

  • Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated.
  • Note that MES and 2,2 Bipyridine have reduced conductivity values. This is because other variables are ions, thus further increase this measurement. This may overwhelm observed effects.

Atomic Absorption Preparation

Creating the Gold Stock Solutions

  • Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  • Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  • Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  • Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  • Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at 4°C were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

Figures

UV-Vis

  • The following UV-Vis are for Lyso 30:1 samples made on March 26, 2014. The samples were synthesized using the method we've been using all year and variable was added added synthesis. The samples were then cooled to 4 Degrees to see the effect on fiber formation.
  • Please note that all for all samples with the exception of the 2,2 Bipyridine samples caused almost all AuNP's to fall out of solution.

Image:Screen_Shot_2014-04-09_at_1.29.09_PM.png Image:Screen_Shot_2014-04-09_at_1.29.34_PM.png Image:Screen_Shot_2014-04-09_at_1.29.45_PM.png

  • Note that due to the measure of accuracy of the equipment used, the percentages that are close in value might not different enough to be considered significant. This is because the values used to calculate these percentages differed by one decimal point ex: .0005 and .0004

Image:Screen_Shot_2014-04-09_at_1.53.20_PM.png


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