User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/09

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===Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C===
===Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C===
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*Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated.
[[Image:4.9.conductivity.4.png|800px|]]
[[Image:4.9.conductivity.4.png|800px|]]
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===Atomic Absorption Preparation===
===Atomic Absorption Preparation===

Revision as of 17:10, 17 April 2014

Biomaterials Design Lab: Spring 2014 Main project page
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Objective

  • Take conductivity measurements on 4°C solutions from 3/26/14.
  • Run UV-Vis and AA on solutions at 36°C from 3/26/14.

Procedure

Conductivity Measurements of 30:1 Lysozyme-AuNP at 4°C

  • Please note that these conductivity values are not corrected for their dilution factor. The true conductivity of these solutions must be further back-calculated.

Atomic Absorption Preparation

Creating the Gold Stock Solutions

  • Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  • Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  • Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  • Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.* Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at 36°C were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

Figures

Atomic Absorption

UV-Vis Images

  • Please note that very few samples actually had suspended nanoparticles in solution.

Image:Screen_Shot_2014-04-15_at_7.37.48_PM.png Image:Screen_Shot_2014-04-15_at_7.39.02_PM.png Image:Screen_Shot_2014-04-15_at_7.39.52_PM.png Image:Screen_Shot_2014-04-16_at_3.04.23_PM.png


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