User:Alexander S Mikheyev/Notebook/bee methylome/2010/04/09

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Library preparation

Reaction mixture conc. 1x
Callow (after bisulfite conversion) ?? 1
PCR primer PE 1.0 ?? 0.2
PCR primer PE 2.0 ?? 0.2
Phusion DNA polymerase 2x 5
MilliQ 3.6
total 10
98C 30 sec
98C 10 sec
65C 30 sec
72C 30 sec
25 cycle
72C 5 min

load 5.0ul of samples



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