User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/02/18

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Alien Genes LongPCR

Amplification of AV212_A, AV240_B, AV_TEL_L_A

' [CONC] 1X 5X
Water 5.3 26.5
Takara LA Taq 5U 0.1 0.5
Takara LA Buffer 10X 1 5
dNTP 2.5uM each 1.6 8
Template 0.5ng 1 5
Primer Mix 5uM each 1
10.0ul 45 / 5 = 9.0ul/ RXN
add 1.0 Primer Mix
0.2ng
*AV 0.5ng
PCR Cycle
94C 1 min
98C 10 sec
68C 15 min
30X
72C 10 min

Loaded 2.0ul of sample

Lane Template Contents ' Fosmid MIX
1 CCA3 AV10016 FWD AV10027RVS AV212_A 5
2 AV AV10016 FWD AV10027RVS AV212_A 5
3 CCA3 AV10041 FWD AV10045 RVS AV240_B 7
4 AV AV10041 FWD AV10045 RVS AV240_B 7
5 CCA3 AV10082 FWD AV10084 RVS AV_TEL_L_A 8
6 AV AV10082 FWD AV10084 RVS AV_TEL_L_A 8
7 CCA3 AV10092 FWD AV10099 RVS AV_TEL_L_A 9
8 AV AV10092 FWD AV10099 RVS AV_TEL_L_A 9
9 CCA3 AV10099 FWD AV10109 RVS AV_TEL_L_A&B 10
10 AV AV10099 FWD AV10109 RVS AV_TEL_L_A&B 10
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