HCO LCO Cycle Sequencing Purification with QIAquick
For Culture 14 - failed injection
http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2
- see page 19.
- 6ul product for cleaning. Resuspended in 10ul EB buffer.
Alien Gene PCR Purification with QIAquick
For previously Ethanol Cleaned samples 09April10
http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2
- see page 19.
- ~8ul PCR product left for cleaning. Resuspended in 30ul EB buffer.
Sample ID
|
Nucleic Acid Conc.
|
Unit
|
A260
|
A280
|
260/280
|
260/230
|
Sample Type
|
Factor
|
1 |
14.5 |
ng/µl |
0.29 |
0.149 |
1.95 |
1.68 |
DNA |
50
|
2 |
12.3 |
ng/µl |
0.245 |
0.122 |
2.01 |
2.21 |
DNA |
50
|
3 |
7.7 |
ng/µl |
0.153 |
0.064 |
2.4 |
1.81 |
DNA |
50
|
4 |
16.3 |
ng/µl |
0.327 |
0.17 |
1.92 |
2.27 |
DNA |
50
|
5 |
11.4 |
ng/µl |
0.228 |
0.109 |
2.09 |
1.79 |
DNA |
50
|
6 |
15 |
ng/µl |
0.3 |
0.152 |
1.97 |
1.83 |
DNA |
50
|
7 |
7.5 |
ng/µl |
0.149 |
0.067 |
2.24 |
2.03 |
DNA |
50
|
8 |
34.8 |
ng/µl |
0.697 |
0.385 |
1.81 |
2.01 |
DNA |
50
|
9 |
30.8 |
ng/µl |
0.617 |
0.341 |
1.81 |
1.99 |
DNA |
50
|
10 |
5.8 |
ng/µl |
0.116 |
0.062 |
1.86 |
1.74 |
DNA |
50
|
11 |
14.2 |
ng/µl |
0.285 |
0.147 |
1.93 |
1.83 |
DNA |
50
|
12 |
10.9 |
ng/µl |
0.219 |
0.102 |
2.15 |
1.86 |
DNA |
50
|
13 |
8.9 |
ng/µl |
0.179 |
0.094 |
1.91 |
2.32 |
DNA |
50
|
14 |
12.9 |
ng/µl |
0.258 |
0.136 |
1.89 |
2.05 |
DNA |
50
|
15 |
44.4 |
ng/µl |
0.889 |
0.489 |
1.82 |
1.8 |
DNA |
50
|
16 |
11.1 |
ng/µl |
0.222 |
0.128 |
1.73 |
1.63 |
DNA |
50
|
17 |
11.5 |
ng/µl |
0.23 |
0.119 |
1.92 |
2.21 |
DNA |
50
|
18 |
11 |
ng/µl |
0.221 |
0.107 |
2.07 |
2.17 |
DNA |
50
|
19 |
6.1 |
ng/µl |
0.122 |
0.061 |
1.99 |
1.71 |
DNA |
50
|
20 |
6.4 |
ng/µl |
0.128 |
0.062 |
2.08 |
2 |
DNA |
50
|
21 |
5.4 |
ng/µl |
0.109 |
0.067 |
1.63 |
1.56 |
DNA |
50
|
22 |
10.2 |
ng/µl |
0.204 |
0.121 |
1.68 |
1.96 |
DNA |
50
|
23 |
15.3 |
ng/µl |
0.306 |
0.175 |
1.75 |
1.56 |
DNA |
50
|
24 |
15.3 |
ng/µl |
0.306 |
0.164 |
1.87 |
1.57 |
DNA |
50
|
25 |
10.3 |
ng/µl |
0.206 |
0.1 |
2.05 |
1.91 |
DNA |
50
|
Sample to add to mix
Sample
|
MIX
|
Contents
|
'
|
Size
|
Gene
|
CCA3 Result
|
Qiagen Cleaned ng/ul
|
Sample Type
|
Total Product Remaining
|
bp/ng
|
ul to mix
|
ng added
|
ul to mix x 5
|
1 |
23 |
AV10004 FWD |
AV10007 RVS |
8691 |
AV212 A |
good 04Mar10 |
14.5 |
DNA |
x 29ul |
0.001668393 |
1.091407102 |
15.82540299 |
5.457035512
|
2 |
1 |
AV10007 FWD |
AV10011 RVS |
9262 |
AV212 A |
good 04Mar10 |
12.3 |
DNA |
x 29ul |
0.001328007 |
1.371149132 |
16.86513433 |
6.855745662
|
3 |
2 |
AV10011 FWD |
AV10016 RVS |
11924 |
AV212 A |
good 02Mar10 |
7.7 |
DNA |
x 29ul |
0.000645756 |
2.81978678 |
21.71235821 |
14.0989339
|
4 |
24 |
AV10016 FWD |
AV10020 RVS |
7020 |
AV212 A |
good 04Mar10 |
16.3 |
DNA |
x 29ul |
0.002321937 |
0.7842139 |
12.78268657 |
3.921069499
|
5 |
25 |
AV10020 FWD |
AV10025 RVS |
11202 |
AV212 A |
good 04Mar10 |
11.4 |
DNA |
x 29ul |
0.001017675 |
1.789269442 |
20.39767164 |
8.946347211
|
6 |
4 |
AV10025 FWD |
AV10028 RVS |
6843 |
AV212 A |
good 04Mar10 |
15 |
DNA |
x 29ul |
0.002192021 |
0.830692537 |
12.46038806 |
4.153462687
|
7 |
34 |
AV10029 FWD |
AV10034 RVS |
12093 |
AV240 B |
good 08Mar10 |
7.5 |
DNA |
x 29ul |
0.000620194 |
2.93601194 |
22.02008955 |
14.6800597
|
8 |
40 |
AV10034 FWD |
AV10039 RVS |
14180 |
AV240 B |
good 18Mar10 |
34.8 |
DNA |
x 29ul |
0.002454161 |
0.741962601 |
25.82029851 |
3.709813004
|
9 |
50 |
AV10039 FWD |
AV10041 FWD |
19555 |
AV240 B |
good 18Mar10 |
30.8 |
DNA |
x 29ul |
0.001575045 |
1.156091297 |
35.60761194 |
5.780456484
|
10 |
30 |
EU643474 FWD |
EU643474 RVS |
10062 |
AV240 B |
good 04Mar10 |
5.8 |
DNA |
x 29ul |
0.000576426 |
3.158939784 |
18.32185075 |
15.79469892
|
11 |
31 |
AV10045 FWD |
AV10047 RVS |
6762 |
AV240 B |
good 04Mar10 |
14.2 |
DNA |
x 29ul |
0.00209997 |
0.867105318 |
12.31289552 |
4.335526592
|
12 |
32 |
AV10047 FWD |
AV10049 RVS |
12719 |
AV240 B |
good 04Mar10 |
10.9 |
DNA |
x 29ul |
0.000856986 |
2.124767904 |
23.15997015 |
10.62383952
|
13 |
33 |
AV10049 FWD |
AV10053 RVS |
9353 |
AV240 B |
good 04Mar10 |
8.9 |
DNA |
x 29ul |
0.000951566 |
1.913577059 |
17.03083582 |
9.567885293
|
14 |
78 |
AV10091 FWD |
AV10092.2 RVS |
8127 |
AV TEL L A |
good 19Mar10 |
12.9 |
DNA |
x 29ul |
0.001587302 |
1.147164179 |
14.79841791 |
5.735820896
|
15 |
85 |
AV10092.2 FWD |
AV10094 RVS |
7036 |
AV TEL L A |
good 01Apr |
44.4 |
DNA |
x 29ul |
0.006310404 |
0.288554525 |
12.8118209 |
1.442772623
|
16 |
16 |
AV10094 FWD |
AV10098 RVS |
8600 |
AV TEL L A |
good 02Mar10 |
11.1 |
DNA |
x 29ul |
0.001290698 |
1.410783918 |
15.65970149 |
7.053919591
|
17 |
134 |
AV10098 FWD |
AV10104 FWD |
8000 |
AV TEL L A&B |
good 01Apr |
11.5 |
DNA |
x 29ul |
0.0014375 |
1.266709929 |
14.56716418 |
6.333549643
|
18 |
51 |
AV10104 RVS |
AV10106 RVS |
9618 |
AV_TEL_L_B |
good 16Mar |
11 |
DNA |
x 29ul |
0.001143689 |
1.59212483 |
17.51337313 |
7.960624152
|
19 |
111 |
AV10106 FWD |
AV10108.2 RVS |
13400 |
AV_TEL_L_B |
good 29Mar |
6.1 |
DNA |
x 29ul |
0.000455224 |
4 |
24.4 |
20
|
20 |
112 |
AV10107.1 FWD |
AV10108 RVS |
7900 |
AV TEL L B |
good 29Mar |
6.4 |
DNA |
x 29ul |
0.000810127 |
2.24766791 |
14.38507463 |
11.23833955
|
21 |
55 |
AV10108 FWD |
AV10111 RVS |
14846 |
AV_TEL_L_B |
good 16Mar |
5.4 |
DNA |
x 29ul |
0.000363734 |
5.006113875 |
27.03301493 |
25.03056938
|
22 |
117 |
AV10111 FWD |
AV10113 RVS |
8400 |
AV TEL L B |
good 29Mar |
10.2 |
DNA |
x 29ul |
0.001214286 |
1.499561018 |
15.29552239 |
7.497805092
|
23 |
128 |
AV10113 FWD |
TEL_L_B_3 RVS |
11500 |
AV TEL L B |
good 01Apr |
15.3 |
DNA |
x 29ul |
0.001330435 |
1.368646961 |
20.94029851 |
6.843234806
|
24 |
131 |
AV10114 FWD |
TEL_L_B_4 RVS |
8500 |
AV TEL L B |
good 01Apr |
15.3 |
DNA |
x 29ul |
0.0018 |
1.011608624 |
15.47761194 |
5.058043118
|
25 |
138 |
AV10083 FWD |
AV10084.2 RVS |
5400 |
AV TEL L A |
good 05Apr10 |
10.3 |
DNA |
x 29ul |
0.001907407 |
0.954644254 |
9.832835821 |
4.773221272
|
|