User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/10: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
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#*(50×10<sup>-3</sup>mol/L)×(0.050L)×(121.14g/mol)=0.3030g of Tris buffer | #*(50×10<sup>-3</sup>mol/L)×(0.050L)×(121.14g/mol)=0.3030g of Tris buffer | ||
#*(50×10<sup>-3</sup>mol/L)×(0.050L)×(58.44g/mol)=0.1461g of NaCl | #*(50×10<sup>-3</sup>mol/L)×(0.050L)×(58.44g/mol)=0.1461g of NaCl | ||
#A stock solution of BSA that was roughly 5 mg in 5 mL in saline was prepared. The actual solution concentration: | |||
#*mass of BSA used=0.00505g; MW of BSA=66430 g/mol | |||
#*(0.00505g/(66430 gmol<sup>-1</sup>))÷0.005L=1.52×10<sup>-5</sup>M BSA | |||
#Ran UV-Vis of the BSA in saline and the saline solution alone between 200 and 800 nm | |||
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Latest revision as of 00:18, 27 September 2017
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Tasks for September 10
Preparing the solutions for UV-VisRefer to Dr. Fox's Lab notebook for full details <br.>
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