User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/24: Difference between revisions
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===Preparation of solutions for HPLC chromatography=== | ===Preparation of solutions for HPLC chromatography=== | ||
To make a solution of 0.5M acetic acid and 0.3M Na<sub>2</sub>SO<sub>4</sub>: | To make a solution of 0.5M acetic acid and 0.3M Na<sub>2</sub>SO<sub>4</sub>: | ||
*(0.5mol HAc/L)(0.5L)(60.05g/mol)(1mL/1.05g)=14.3mL of acetic acid need to be added under hood to 500 mL volumetric flask | *(0.5mol HAc/L)(0.5L)(60.05g/mol)(1mL/1.05g)=14.3mL of acetic acid need to be added (under fume hood) to 500 mL volumetric flask | ||
*(0.3mol NaSO<sub>4</sub>/L)(0.5L)(142.04g/mol)=21.3060 g of NaSO<sub>4</sub> need to be added to volumetric flask | *(0.3mol NaSO<sub>4</sub>/L)(0.5L)(142.04g/mol)=21.3060 g of NaSO<sub>4</sub> need to be added to volumetric flask | ||
*Make up to 500 mL with HPLC water | *Make up to 500 mL with HPLC water |
Revision as of 09:55, 24 September 2014
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Tasks for September 24To run HPLC with a GPC column Preparation of solutions for HPLC chromatographyTo make a solution of 0.5M acetic acid and 0.3M Na2SO4:
To make dilutions of 1g/L of lsozyme:
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