User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/30: Difference between revisions
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#Ca<sup>2+</sup> calibration curve | #Ca<sup>2+</sup> calibration curve | ||
#*Prepare 25 mL 50 mM CaCl<sub>2</sub> using HPLC water | #*Prepare 25 mL 50 mM CaCl<sub>2</sub> using HPLC water | ||
#**Added 0.13875g CaCl<sub>2</sub> to a 25 mL volumetric flask and made up to the mark with HPLC | |||
#**Added 0.0730 g NaCl to a 25 mL volumetric flask and made up to the mark with HPLC | #**Added 0.0730 g NaCl to a 25 mL volumetric flask and made up to the mark with HPLC | ||
#*Using serial dilutions, prepare 25 mL each of 5 mM, 500 μM, 50 μM, & 5 μM CaCl<sub>2</sub> | #*Using serial dilutions, prepare 25 mL each of 5 mM, 500 μM, 50 μM, & 5 μM CaCl<sub>2</sub> |
Revision as of 09:51, 30 September 2014
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Tasks for September 30
Making up calibration curves for Ca2+ and Cl-Procedure followed as described by Dr. Fox
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