User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/30

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Tasks for September 30

  • To run dyalisis
  • To prepare calibration curves Ca2+ and Cl- calibration curves

Making up calibration curves for Ca2+ and Cl-

Procedure followed as described by Dr. Fox

  1. Ca2+ calibration curve
    • Prepare 25 mL 50 mM CaCl2 using HPLC water
    • Using serial dilutions, prepare 25 mL each of 5 mM, 500 μM, 50 μM, & 5 μM CaCl2
    • Measure Ca2+ using ISE
    • Try measuring 50 μM and 50 mM of other salts to check for interferences (Mg2+ should, Na+ should not)
    • Be sure to measure HPLC water, DI water, tap water, and your lysozyme stock solution.
  2. Cl- calibration curve
    • Prepare 25 mL 50 mM NaCl using HPLC water
    • Using serial dilutions, prepare 25 mL each of 5 mM, 500 μM, 50 μM, & 5 μM NaCl
    • Measure Cl- using ISE
    • Try measuring 50 μM and 50 mM of other salts to check for interferences & ion-pairing
    • Be sure to measure HPLC water, DI water, tap water, and your lysozyme stock solution.
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