User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/08: Difference between revisions
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*** 12.5 mL NH<sub>4</sub>SCN used | *** 12.5 mL NH<sub>4</sub>SCN used | ||
** 2 mM KI against Lysozyme | ** 2 mM KI against Lysozyme | ||
*** mL NH<sub>4</sub>SCN used | *** 13.6 mL NH<sub>4</sub>SCN used | ||
** 5 mM KI against Lysozyme | ** 5 mM KI against Lysozyme | ||
*** mL NH<sub>4</sub>SCN used | *** 13.4 mL NH<sub>4</sub>SCN used | ||
** 10 mM KI against Lysozyme | ** 10 mM KI against Lysozyme | ||
*** | *** 13.2 mL NH<sub>4</sub>SCN used | ||
** 25 mM KI against Lysozyme | ** 25 mM KI against Lysozyme | ||
*** mL NH<sub>4</sub>SCN used | *** 12.6 mL NH<sub>4</sub>SCN used | ||
** 50 mM KI against Lysozyme | ** 50 mM KI against Lysozyme | ||
*** mL NH<sub>4</sub>SCN used | *** 11.9 mL NH<sub>4</sub>SCN used | ||
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Revision as of 11:39, 21 October 2014
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Tasks for October 8
[I-] by Titration PrecipitationProcedure followed as detailed by Dr. Fox
Fluorescence of protein solutions
Preparation of new Dialysis with 0.6 g/ L Lysozyme vs KI using 3500 MWCO
Dialysis DataBradford Analysis
Bradford Calibration curve
<br.> UV-Vis Absorption <br.> Transfer 100 μL to a small volume UV cuvette & measure UV absorption
Fluorescence
[I-] titration
Titrations were performed as follows
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