User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/15: Difference between revisions
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*** Well 6: 0.6 g/L Lysozyme | *** Well 6: 0.6 g/L Lysozyme | ||
** Run as previously described. Refer to [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 Dr. Harting's protocol]. | ** Run as previously described. Refer to [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 Dr. Harting's protocol]. | ||
===Determination of molecular weight=== | |||
*By comparison with Precision Plus Protein Standards, the molecular weight of all samples was close to 15 KD, slightly lower, as expected for lysozyme compounds with MW=14,300 KD. | |||
* There was a very small quantity of unknown A and colloid in the gel. The very faint mark observed in well 3 could even be considered to be bleed-over from the 30:1 colloid in well 4. Therefore, electrophoresis was inclonclusive with respect to the molecular weight of unknown A. | |||
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Revision as of 10:00, 21 October 2014
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Tasks for October 15
SDS-PAGE
Determination of molecular weight
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