User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/28: Difference between revisions
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===0.6 g/L lysozyme vs CaCl<sub>2</sub> using 3500 MWCO dialysis data=== | ===0.6 g/L lysozyme vs CaCl<sub>2</sub> using 3500 MWCO dialysis data=== | ||
<u>Bradford Analysis</u> | |||
*Only running bradford analysis of protein-containing solutions, that is Lys 1, 2, 3, 4 and 5, since the protein cannot diffuse through 3500 MWCO. | |||
* Remove 50 μL of solution from each chamber (7 in all) and run Bradford analysis | |||
**Bradford reagent should be diluted 1:3 with 50mM Tris/50mM NaCl | |||
**Recall, 50 μL solution + 200 μL diluted Bradford + 750 μL Tris/NaCl buffer | |||
**PS cuvettes, measuring 400 - 800 nm | |||
<u>Ca2+ ISE</u> | <u>Ca2+ ISE</u> | ||
{| {{table}} | {| {{table}} |
Revision as of 12:05, 29 October 2014
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Tasks for October 28
I- ISE
0.6 g/L lysozyme vs CaCl2 using 3500 MWCO dialysis dataBradford Analysis
Ca2+ ISE
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