User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/10/29

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Tasks for October 29

  • Dialysis of 0.6 g/L Lysozyme vs HCl using MWCO 20000

Preparation of HCl stock solutions

  • Need to prepare HCl stock solution of 75 μM, 100 μM, 250 μM, 500 μM and 5 mM with distilled water.
    • The initial stock solution of 5 mM HCl was prepared by diluting 0.5 mL of 0.5 N HCl to 50 mL using distilled water
      • 0.5v1=5×10-3(50mL); v1=0.5 mL.
    • Made remaining stock solutions from 5 mM stock

Preparation of new 0.g/L lysozyme stock

  • Had to prepare new 0.6 g/L lysozyme since it precipitated out of solution.
  • Made 50 mL of 0.6 g/L lysozyme solution by adding 0.0380 g of lysozyme (Theoretical value 0.030 g, but some may have been lost in transfer).
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Alicia_Rasines_Mazo/Notebook/CHEM-571_Experimental_Biological_Chemistry/2014/10/29&oldid=839910"