User:Alicia Rasines Mazo/Notebook/CHEM-572 Experimental Biological Chemistry II/2015/01/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<u>30:1 AuLys colloid</u> | <u>30:1 AuLys colloid</u> | ||
*Per mL test tube, add 0.5 mL HAuCl<sub>4</sub> stock solution (final concentration 251 μM) | *Per mL test tube, add 0.5 mL HAuCl<sub>4</sub> stock solution (final concentration 251 μM) | ||
* Also add 2. | * Also add 2.095 mL of 19.81μM of lysozyme stock | ||
**m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | **m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | ||
**8.3×10<sup>-6</sup>(5mL)=19.81×10<sup>-6</sup>v<sub>2</sub> | **8.3×10<sup>-6</sup>(5mL)=19.81×10<sup>-6</sup>v<sub>2</sub> | ||
**v<sub>2</sub>=2.095 mL | **v<sub>2</sub>=2.095 mL | ||
*Make up to 5 mL with distilled water | *Make up to 5 mL with distilled water (2.405 mL) | ||
*Repeat for 9 other test tubes | |||
<u>45:1 AuLys fibers</u> | <u>45:1 AuLys fibers</u> | ||
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**5.56×10<sup>-6</sup>(5mL)=19.81×10<sup>-6</sup>v<sub>2</sub> | **5.56×10<sup>-6</sup>(5mL)=19.81×10<sup>-6</sup>v<sub>2</sub> | ||
**v<sub>2</sub>=1.40 mL | **v<sub>2</sub>=1.40 mL | ||
*Make up to 5 mL with distilled water | *Make up to 5 mL with distilled water (3.1 mL) | ||
*Repeat for 9 other test tubes | |||
Place in oven for 4h at 80°C. | |||
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__NOTOC__ | __NOTOC__ |
Latest revision as of 00:41, 27 September 2017
Project name | Main project page Next entry |
Tasks for January 27
Preparation of stock solutions
Note: The pH of the solution has to be kept at 3.6 for optimum formation of AuNPs. Preparation of Au colloid and fibers30:1 AuLys colloid
45:1 AuLys fibers
Place in oven for 4h at 80°C. |