User:Alicia Rasines Mazo/Notebook/CHEM-572 Experimental Biological Chemistry II/2015/01/27: Difference between revisions

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* Formation of 30:1 Gold-Lysozyme nanoparticle colloid
* Formation of 30:1 Gold-Lysozyme nanoparticle colloid
* Formation of 45:1 Gold-Lysozyme fibers
* Formation of 45:1 Gold-Lysozyme fibers
===Preparation of stock solutions===
*50 mL of 2.512mM HAuCl4 stock solution was prepared by adding 0.0427 g of HAuCl4 in distilled water.
**(0.05L)(2.512×10<sup>-3</sup> mol/L)(340 g/mol)=0.0427 g
*50 mL of 20 μM lysozyme stock solution was prepared by adding 0.014307 g of Lysozyme in distilled water
**((0.05L)(20×10<sup>-6</sup> mol/L)(14307 g/mol)= 0.014307 g
Note: The pH of the solution has to be kept at 3.6 for optimum formation of AuNPs.
*Per mL test tube, add 0.5 mL HAuCl<sub>4</sub> stock solution (final concentration 250 μM)
* Also add 2.075 mL of 20μM of lysozyme stock
**m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
**8.3×10<sup>-6</sup>(5mL)=20×10<sup>-6</sup>v<sub>2</sub>
**v<sub>2</sub>=2.075 mL
*Make up to 5 mL with distilled water




Revision as of 11:23, 27 January 2015

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Tasks for January 27

  • Formation of 30:1 Gold-Lysozyme nanoparticle colloid
  • Formation of 45:1 Gold-Lysozyme fibers

Preparation of stock solutions

  • 50 mL of 2.512mM HAuCl4 stock solution was prepared by adding 0.0427 g of HAuCl4 in distilled water.
    • (0.05L)(2.512×10-3 mol/L)(340 g/mol)=0.0427 g
  • 50 mL of 20 μM lysozyme stock solution was prepared by adding 0.014307 g of Lysozyme in distilled water
    • ((0.05L)(20×10-6 mol/L)(14307 g/mol)= 0.014307 g

Note: The pH of the solution has to be kept at 3.6 for optimum formation of AuNPs.

  • Per mL test tube, add 0.5 mL HAuCl4 stock solution (final concentration 250 μM)
  • Also add 2.075 mL of 20μM of lysozyme stock
    • m1v1=m2v2
    • 8.3×10-6(5mL)=20×10-6v2
    • v2=2.075 mL
  • Make up to 5 mL with distilled water



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