Tasks for January 27
- Formation of 30:1 Gold-Lysozyme nanoparticle colloid
- Formation of 45:1 Gold-Lysozyme fibers
Preparation of stock solutions
- 100 mL of 2.512mM HAuCl4 stock solution was prepared by adding 0.085 g of HAuCl4 in distilled water.
- (0.1L)(2.512×10-3 mol/L)(340 g/mol)=0.085 g (0.08533 g were weighed out)
- 50 mL of 20 μM lysozyme stock solution was prepared by adding 0.014307 g of Lysozyme in distilled water
- ((0.05L)(20×10-6 mol/L)(14307 g/mol)= 0.014307 g (0.01417g were weighed out)
Note: The pH of the solution has to be kept at 3.6 for optimum formation of AuNPs.
Preparation of Au colloid and fibers
30:1 AuLys colloid
- Per mL test tube, add 0.5 mL HAuCl4 stock solution (final concentration 251 μM)
- Also add 2.095 mL of 19.81μM of lysozyme stock
- m1v1=m2v2
- 8.3×10-6(5mL)=19.81×10-6v2
- v2=2.095 mL
- Make up to 5 mL with distilled water (2.405 mL)
- Repeat for 9 other test tubes
45:1 AuLys fibers
- Per mL test tube, add 0.5 mL HAuCl4 stock solution (final concentration 251 μM)
- Also add 1.40 mL of 19.81μM of lysozyme stock
- m1v1=m2v2
- 5.56×10-6(5mL)=19.81×10-6v2
- v2=1.40 mL
- Make up to 5 mL with distilled water (3.1 mL)
- Repeat for 9 other test tubes
Place in oven for 4h at 80°C.
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