User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/09/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* To continue processing films from last week | * To continue processing films from last week | ||
* To make new films with exfoliated clay | * To make new films with exfoliated clay | ||
* To run AA on the arsenic samples | * To run AA on the arsenic samples | ||
* To measure fluorescence of Rhodamine dye | |||
===Preparation of 10% wt new exfoliated clay films=== | ===Preparation of 10% wt new exfoliated clay films=== | ||
*1.0042 g 22,000 PVA, 0.1101 g of of exfoliated tributylhexadecylphosphonium bromide (THP Br) and 7 mL of distilled water were used to make up a 10% wt PVA-exfoliated THP Br film. Note this clay was prepared by Jame's group. | *1.0042 g 22,000 PVA, 0.1101 g of of exfoliated tributylhexadecylphosphonium bromide (THP Br) and 7 mL of distilled water were used to make up a 10% wt PVA-exfoliated THP Br film. Note this clay was prepared by Jame's group. | ||
*1.0028 g 22,000 PVA, 0.1107 g of exfoliated tributylhexadecylphosphonium bromide (THP Br) and 7 mL of distilled water were used to make up a 10% wt PVA-exfoliated THP Br film. | *1.0028 g 22,000 PVA, 0.1107 g of exfoliated tributylhexadecylphosphonium bromide (THP Br) and 7 mL of distilled water were used to make up a 10% wt PVA-exfoliated THP Br film. | ||
*Heated at | *Heated at approx. 80ºC with gentle stirring until all crystals were dissolved | ||
*Allowed to cool down to room temeprature | |||
*Added 0.8 wt% 0.5 mL glutaraldehyde under hood | *Added 0.8 wt% 0.5 mL glutaraldehyde under hood | ||
*Stirred for 3 min to ensure homogeneity | *Stirred for 3 min to ensure homogeneity | ||
*Poured into teflon dish and allowed to dry | *Poured into teflon dish and allowed to dry | ||
===Completing synthesis of films=== | ===Completing synthesis of films=== | ||
*Soaked in sodium sulfate for 1h, | *Soaked in sodium sulfate for 1h, put into 0.1% HCl for 30 min, then sodium bicarbonate solution for a further 15 min. | ||
===Run AA on As samples=== | ===Run AA on As samples=== | ||
*James and Eleni made made As standards: 0, 10, 20, 30, 40 and 50 ppm. | *James and Eleni made made As standards: 0, 10, 20, 30, 40 and 50 ppm. | ||
*Diluted 100 and 150 ppm As sample solutions so that: | *Diluted the 100 and 150 ppm As sample solutions so that: | ||
**150ppm(v<sub>1</sub>)=30ppm(10mL) | **150ppm(v<sub>1</sub>)=30ppm(10mL) | ||
**v<sub>1</sub>=2 mL of 150 ppm As solution. | **v<sub>1</sub>=2 mL of 150 ppm As solution. To be diluted to 10 mL with distilled water. | ||
**100ppm(v<sub>1</sub>)=25ppm(10mL) | **100ppm(v<sub>1</sub>)=25ppm(10mL) | ||
**v<sub>1</sub>=2.5 mL of 100 ppm solution. | **v<sub>1</sub>=2.5 mL of 100 ppm solution. To be diluted to 10 mL with distilled water. | ||
*Note: 50 ppm As solutions were left undiluted, as they were within the range of the range calibration curve | |||
'''To be done at a later date since As lamp was burnt out''' | |||
===Measuring fluorescence of Rhodamine dye by films=== | |||
<span style="color:red">Note: the initial concentration of rhodamine was incorrectly labeled as 159μM, actual concentration was 1.9 mM</span><br.> | |||
Solutions were made for 20, 10 and 1 μM R6G by: | |||
*Adding 12.578 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''20μM R6G'''. | |||
*Adding 6.289 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''10 μM R6G'''. | |||
*Adding 0.629 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''1μM R6G'''. | |||
*<span style="color:red">Corrections:</span> | |||
*Adding 12.578 mL of 1.9 mM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up <span style="color:red">'''239.4 μM'''</span> | |||
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye. | |||
*Adding 6.289 mL of 1.9 mM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up <span style="color:red">'''119.7 μM'''</span> | |||
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye. | |||
*Adding 0.629 mL of 1.9 mM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up <span style="color:red">'''11.97 μM'''</span> | |||
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Film''' | |||
| align="center" style="background:#f0f0f0;"|'''Solution''' | |||
| align="center" style="background:#f0f0f0;"|'''Vial mass (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Vial + film mass (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Film mass (g)''' | |||
|- | |||
| PVA film|| <span style="color:red">239.4 μM</span> R6G|| 13.0803 || 13.1813 || 0.1010 | |||
|- | |||
| || <span style="color:red"> 119.7 μM</span> R6G|| 13.0713 || 13.1497 || 0.0784 | |||
|- | |||
| || <span style="color:red">11.97 μM</span> R6G||13.0403 || 13.1337 || 0.0934 | |||
|- | |||
| PVA-Clay film|| <span style="color:red">239.4 μM</span> R6G|| 13.0828 || 13.2554 || 0.1726 | |||
|- | |||
| || <span style="color:red">119.7 μM</span> R6G|| 12.9552 || 13.0732 || 0.1180 | |||
|- | |||
| || <span style="color:red">11.97 μM</span> R6G|| 13.0198 || 13.1377 || 0.1179 | |||
|} | |||
===Measuring absorbance of R6G dye by polymer and clay films=== | |||
* UV-Vis Results | |||
**PVA film | |||
{| {{table}} | |||
|- | |||
! PVA in | |||
! R6G dye left in solution (μM) | |||
! R6G dye absorbed by film (μM) | |||
|- | |||
| 239.4 μM R6G | |||
| 254.3* (Retake measurement) | |||
| | |||
|- | |||
| 119.7 μM R6G | |||
| 53.8 | |||
| 65.9 | |||
|- | |||
| 11.97 μM R6G | |||
| 1.076 | |||
| 10.894 | |||
|- | |||
|} | |||
10 mL of R6G solution were added to each vial, so that if 10.894 μM were absorbed from the 11.97 μM solution, moles absorbed=(10.894×10<sup>-6</sup>moles)/0.010L=0.0010894 moles. | |||
*mass of R6G dye absorbed=0.0010894 moles × 479.02g/mol=0.5218g | |||
*11.97 μM: (0.5218 g R6G/0.0934 film)=''' 5.59 g R6G/g film''' | |||
*119.7 μM: ''' 40.3 g R6G/g film''' | |||
*239.4 μM: | |||
**PVA-Clay film | |||
{| {{table}} | |||
|- | |||
! PVA-Clay in | |||
! R6G dye left in solution (μM) | |||
! R6G dye absorbed by film (μM) | |||
|- | |||
| 239.4 μM R6G | |||
| 38.79 | |||
| 200.6 | |||
|- | |||
| 119.7 μM R6G | |||
| 15.09 | |||
| 104.6 | |||
|- | |||
| 11.97 μM R6G | |||
| 0.776 | |||
| 11.19 | |||
|- | |||
|} | |||
10 mL of R6G solution were added to each vial, so that if 11.194 μM were absorbed from the 11.97 μM solution, moles absorbed=(11.194×10<sup>-6</sup>moles)/0.010L=0.0011194 moles. | |||
*mass of R6G dye absorbed=0.0011194 moles × 479.02g/mol=0.5362 g | |||
*11.97 μM: (0.5362 g R6G/0.1179 film)=''' 4.55 g R6G/g film''' | |||
*119.7 μM: ''' 42.5 g R6G/g film''' | |||
*239.4 μM: ''' 55.7 g R6G/g film''' | |||
*Fluorescence data | |||
**PVA film | |||
{| {{table}} | |||
|- | |||
! PVA in | |||
! R6G dye left in solution (μM) | |||
! R6G dye absorbed by film (μM) | |||
|- | |||
| 239.4 μM R6G | |||
| 227.9 | |||
| 11.5 | |||
|- | |||
| 119.7 μM R6G | |||
| 39.28 | |||
| 80.42 | |||
|- | |||
| 11.97 μM R6G | |||
| 1.525 | |||
| 10.445 | |||
|- | |||
|} | |||
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__NOTOC__ | __NOTOC__ |
Latest revision as of 00:20, 27 September 2017
Project name | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Tasks for September 24
Preparation of 10% wt new exfoliated clay films
Completing synthesis of films
Run AA on As samples
To be done at a later date since As lamp was burnt out Measuring fluorescence of Rhodamine dye by filmsNote: the initial concentration of rhodamine was incorrectly labeled as 159μM, actual concentration was 1.9 mM<br.> Solutions were made for 20, 10 and 1 μM R6G by:
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye.
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye.
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye.
Measuring absorbance of R6G dye by polymer and clay films
10 mL of R6G solution were added to each vial, so that if 10.894 μM were absorbed from the 11.97 μM solution, moles absorbed=(10.894×10-6moles)/0.010L=0.0010894 moles.
10 mL of R6G solution were added to each vial, so that if 11.194 μM were absorbed from the 11.97 μM solution, moles absorbed=(11.194×10-6moles)/0.010L=0.0011194 moles.
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