User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/09/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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'''To be done at a later date since As lamp was burnt out''' | '''To be done at a later date since As lamp was burnt out''' | ||
===Measuring fluorescence of Rhodamine dye by films=== | ===Measuring fluorescence of Rhodamine dye by films=== | ||
<span style="color:red">Note: the initial concentration of rhodamine was incorrectly labeled as 159μM, actual concentration was 1.9 mM</span><br.> | |||
Solutions were made for 20, 10 and 1 μM R6G by: | Solutions were made for 20, 10 and 1 μM R6G by: | ||
*Adding 12.578 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''20μM R6G'''. | *Adding 12.578 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''20μM R6G'''. | ||
*Adding 6.289 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''10 μM R6G'''. | *Adding 6.289 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''10 μM R6G'''. | ||
*Adding 0.629 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''1μM R6G'''. | *Adding 0.629 mL of 159μM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up '''1μM R6G'''. | ||
*<span style="color:red">Corrections:</span> | |||
*Adding 12.578 mL of 1.9 mM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up <span style="color:red">'''239.4 μM'''</span> | |||
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye. | |||
*Adding 6.289 mL of 1.9 mM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up <span style="color:red">'''119.7 μM'''</span> | |||
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye. | |||
*Adding 0.629 mL of 1.9 mM rhodamine dye solution to a 100 mL volumetric flask and making up to the mark with distilled water to make up <span style="color:red">'''11.97 μM'''</span> | |||
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye. | 10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye. | ||
{| {{table}} | {| {{table}} | ||
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| align="center" style="background:#f0f0f0;"|'''Film mass (g)''' | | align="center" style="background:#f0f0f0;"|'''Film mass (g)''' | ||
|- | |- | ||
| PVA film|| | | PVA film|| <span style="color:red">239.4 μM</span> R6G|| 13.0803 || 13.1813 || 0.1010 | ||
|- | |||
| || <span style="color:red"> 119.7 μM</span> R6G|| 13.0713 || 13.1497 || 0.0784 | |||
|- | |||
| || <span style="color:red">11.97 μM</span> R6G||13.0403 || 13.1337 || 0.0934 | |||
|- | |||
| PVA-Clay film|| <span style="color:red">239.4 μM</span> R6G|| 13.0828 || 13.2554 || 0.1726 | |||
|- | |||
| || <span style="color:red">119.7 μM</span> R6G|| 12.9552 || 13.0732 || 0.1180 | |||
|- | |||
| || <span style="color:red">11.97 μM</span> R6G|| 13.0198 || 13.1377 || 0.1179 | |||
|} | |||
===Measuring absorbance of R6G dye by polymer and clay films=== | |||
* UV-Vis Results | |||
**PVA film | |||
{| {{table}} | |||
|- | |||
! PVA in | |||
! R6G dye left in solution (μM) | |||
! R6G dye absorbed by film (μM) | |||
|- | |||
| 239.4 μM R6G | |||
| 254.3* (Retake measurement) | |||
| | |||
|- | |||
| 119.7 μM R6G | |||
| 53.8 | |||
| 65.9 | |||
|- | |- | ||
| | | 11.97 μM R6G | ||
| 1.076 | |||
| 10.894 | |||
|- | |- | ||
| | |} | ||
10 mL of R6G solution were added to each vial, so that if 10.894 μM were absorbed from the 11.97 μM solution, moles absorbed=(10.894×10<sup>-6</sup>moles)/0.010L=0.0010894 moles. | |||
*mass of R6G dye absorbed=0.0010894 moles × 479.02g/mol=0.5218g | |||
*11.97 μM: (0.5218 g R6G/0.0934 film)=''' 5.59 g R6G/g film''' | |||
*119.7 μM: ''' 40.3 g R6G/g film''' | |||
*239.4 μM: | |||
**PVA-Clay film | |||
{| {{table}} | |||
|- | |||
! PVA-Clay in | |||
! R6G dye left in solution (μM) | |||
! R6G dye absorbed by film (μM) | |||
|- | |||
| 239.4 μM R6G | |||
| 38.79 | |||
| 200.6 | |||
|- | |||
| 119.7 μM R6G | |||
| 15.09 | |||
| 104.6 | |||
|- | |||
| 11.97 μM R6G | |||
| 0.776 | |||
| 11.19 | |||
|- | |||
|} | |||
10 mL of R6G solution were added to each vial, so that if 11.194 μM were absorbed from the 11.97 μM solution, moles absorbed=(11.194×10<sup>-6</sup>moles)/0.010L=0.0011194 moles. | |||
*mass of R6G dye absorbed=0.0011194 moles × 479.02g/mol=0.5362 g | |||
*11.97 μM: (0.5362 g R6G/0.1179 film)=''' 4.55 g R6G/g film''' | |||
*119.7 μM: ''' 42.5 g R6G/g film''' | |||
*239.4 μM: ''' 55.7 g R6G/g film''' | |||
*Fluorescence data | |||
**PVA film | |||
{| {{table}} | |||
|- | |||
! PVA in | |||
! R6G dye left in solution (μM) | |||
! R6G dye absorbed by film (μM) | |||
|- | |||
| 239.4 μM R6G | |||
| 227.9 | |||
| 11.5 | |||
|- | |- | ||
| | | 119.7 μM R6G | ||
| 39.28 | |||
| 80.42 | |||
|- | |- | ||
| | | 11.97 μM R6G | ||
| 1.525 | |||
| 10.445 | |||
|- | |- | ||
|} | |} | ||
Latest revision as of 00:20, 27 September 2017
Project name | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Tasks for September 24
Preparation of 10% wt new exfoliated clay films
Completing synthesis of films
Run AA on As samples
To be done at a later date since As lamp was burnt out Measuring fluorescence of Rhodamine dye by filmsNote: the initial concentration of rhodamine was incorrectly labeled as 159μM, actual concentration was 1.9 mM<br.> Solutions were made for 20, 10 and 1 μM R6G by:
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye.
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye.
10mL of each R6G dye solution were added to the PVA and PVA-Clay glass vials and the glass vials were placed inside a dark drawer to allow the films to soak the fluorescent dye.
Measuring absorbance of R6G dye by polymer and clay films
10 mL of R6G solution were added to each vial, so that if 10.894 μM were absorbed from the 11.97 μM solution, moles absorbed=(10.894×10-6moles)/0.010L=0.0010894 moles.
10 mL of R6G solution were added to each vial, so that if 11.194 μM were absorbed from the 11.97 μM solution, moles absorbed=(11.194×10-6moles)/0.010L=0.0011194 moles.
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