User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/10/01: Difference between revisions
From OpenWetWare
No edit summary |
(fix raw html notebook nav) |
||
(6 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 18: | Line 18: | ||
## 1.5μM | ## 1.5μM | ||
## 2.0μM | ## 2.0μM | ||
#* Due to extremely low amounts of R6G required to make stock solutions, an initial stock solution of 2500 μM was prepared | #* Due to extremely low amounts of R6G required to make stock solutions, an initial stock solution of 2500 μM was prepared. This solution was further diluted to 500μM to make rest of solutions, except for 2.0 μM, which was prepared using 2500μM stock solution | ||
#**(2500×10<sup>-6</sup>mol/L)*(0.010L)*(474.02g/mol)=0.01185 g ('''0.0120 g of R6G were measured out) | #**(2500×10<sup>-6</sup>mol/L)*(0.010L)*(474.02g/mol)=0.01185 g ('''0.0120 g of R6G were measured out) | ||
#*** 2μM stock concentration: (2500μM)v<sub>1</sub>=(2.0μM)(5mL); '''v<sub>1</sub>'''=0.004mL | #*** 2μM stock concentration: (2500μM)v<sub>1</sub>=(2.0μM)(5mL); '''v<sub>1</sub>'''=0.004mL | ||
Line 38: | Line 38: | ||
## In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height. | ## In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height. | ||
===Calibration curve of R6G using UV-Vis=== | ===Calibration curve of R6G using UV-Vis=== | ||
[[Image:R6G UVVIS calibration.png]]<br.> | |||
[[Image:MolarAbsorp R6G 521.png]] | |||
===Calibration curve of R6G based on fluorescence=== | ===Calibration curve of R6G based on fluorescence=== | ||
[[Image:Fluorescence R6G.png]]<br.> | |||
[[Image:Fluorescence R6G calibrationcurve.png]] | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 00:21, 27 September 2017
Project name | Main project page Previous entry Next entry |
Task for October 1
FluorescenceProcedure followed as directed by Dr. Hartings on Oct. 1<br.>
Calibration curve of R6G using UV-VisCalibration curve of R6G based on fluorescence |