User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/10/01: Difference between revisions
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#* Due to extremely low amounts of R6G required to make stock solutions, an initial stock solution of 2500 μM was prepared, this solution was further diluted to 500μM to make rest of solutions, except for 2.0 μM, which was prepared using 2500μM stock solution | #* Due to extremely low amounts of R6G required to make stock solutions, an initial stock solution of 2500 μM was prepared, this solution was further diluted to 500μM to make rest of solutions, except for 2.0 μM, which was prepared using 2500μM stock solution | ||
#**(2500×10<sup>-6</sup>mol/L)*(0.010L)*(474.02g/mol)=0.01185 g ('''0.0120 g of R6G were measured out) | #**(2500×10<sup>-6</sup>mol/L)*(0.010L)*(474.02g/mol)=0.01185 g ('''0.0120 g of R6G were measured out) | ||
#*** 2μM stock concentration: (2500μM)v<sub>1</sub>=(2.0μM)(5mL); '''v<sub>1</sub>'''=0.004mL | |||
# Take UV-Vis and Fluorescence spectra of these samples. | # Take UV-Vis and Fluorescence spectra of these samples. | ||
# Make a calibration curve based on UV-Vis. | # Make a calibration curve based on UV-Vis. |
Revision as of 05:48, 1 October 2014
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Task for October 1
FluorescenceProcedure followed as directed by Dr. Hartings on Oct. 1<br.>
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