User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/10/01

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Task for October 1

  • To determine fluorophore concentration

Fluorescence

Procedure followed as directed by Dr. Hartings on Oct. 1<br.>

  1. Made stock concentrations of Rhodamine 6G (both groups can use the same solutions)
    1. 0.10μM
    2. 0.50μM
    3. 1.0μM
    4. 1.5μM
    5. 2.0μM
    • Due to extremely low amounts of R6G required to make stock solutions, an initial stock solution of 2500 μM was prepared, rest of solutions were diluted from 2500μM.
      • (2500×10-6mol/L)*(0.010L)*(474.02g/mol)=0.01185 g (0.0120 g of R6G were measured out)
  2. Take UV-Vis and Fluorescence spectra of these samples.
  3. Make a calibration curve based on UV-Vis.
    1. Compare your data to some published values
  4. Make a calibration curve based on the fluorescence.
    1. In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.


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