User:Alison D. Bunnen/Notebook/Biology 210 at AU

1/28/15 Lab 3: Microbiology and Identifying Bacteria with DNA Sequences

I. Purpose:

II. Materials and Methods:

III. Data and Observations:

IV. Conclusions:

1/21/15 Lab 2: Identifying Algae and Protists

I. Purpose: The purpose of this lab is to examine and understand the characteristics of algae and protists from my transect.

II. Materials and Methods:

· Make a wet mount from the niche at the top of the jar and make a wet mount from the niche at the bottom of the jar

· View at 4x, 10x, and 40x.

· Make observations of at least 3 different organisms from each niche

· Use a dichotomous key to identify the organisms

III. Data and Observations:

IV. Conclusions:

--Alison D. Bunnen 18:33, 29 January 2015 (EST)

1/14/15 Lab 1: Biological Life at AU

I. Purpose: The purpose of today's lab was to observe and record characteristics of an assigned transect. We took samples from our niche to create a Hay Infusion Culture. I hypothesize the transects have copious amounts of bacteria living in the niche. If water is added to the gathered materials and is allowed to incubate, then the bacteria from the niche (in the soil) will grow in the new ecosystem created.

II. Materials and Methods:

· 20 x 20 meter transect to study

· Use a sterile 50 mL conical tube to gather materials such as soil and vegetation from transect

· Place 10-12 grams of gathered materials into a large jar

· Add 500 mLs of deerpark water to the jar

· Add 0.1 gm of dried milk into the jar and screw on lid

· Mix contents gently for 10 seconds

· Place the jar in designated lab section area with the jar labeled and lid off

III. Data and Observations: My assigned transect was group 3: Tall Bushes. I recorded macroscopic observations of abiotic and biotic components in my transect. Then my group made a Hay Infusion Culture from materials gathered from our niche. The Hay Infusion Culture was a mix of ~10 grams of soil and vegetation from my transect, 500 mL of deerpark water, and 0.1 gm of dried milk.

The abiotic components inside the transect included:

· Light

· Water/snow

· Concrete

· A metal lamp post

· Dirt

· Rocks

The biotic components inside the transect included:

· Grass

· Shrub

· Bushes

Figure 1.

Figure 2.

Figure 3.

Figure 1. is an aerial-view diagram of my transect labeled with it's abiotic and biotic components.

Figure 2. is a satellite view of Group 3's transect.

Figure 3. is a map view of Group 3's transect.

IV. Conclusions: There are abiotic and biotic components in Group 3's transect. I will not know if my hypothesis is correct until further examination of the incubated Hay Infusion Culture is done.

--Alison D. Bunnen 22:24, 26 January 2015 (EST)