User:Alji: Difference between revisions
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! Overlapping Areas !! Modified sequence | ! Overlapping Areas !! Original Sequence !! Modified sequence | ||
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| Gene 2 ORF and Gene 10 RBS || || | | Gene 2 ORF and Gene 10 RBS || || | ||
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The refactored genome is [] on the Registry of Standard Biological Parts | The refactored genome is [Part BBa_M31332 | http://parts.mit.edu/registry/index.php/Part:BBa_M31332] on the Registry of Standard Biological Parts |
Revision as of 10:19, 28 February 2007
Andrew Ji
About Me
- Year: 2009
- Major: Biological Engineering
- Minor: Economics
- Email: alji AT mit DOT edu
M13K07 Genome Re-engineering ideas:
Gene | Ideas |
---|---|
I | Change size of protein to see effect of different channel sizes. |
II | Increase or decrease expression to optimize rate of + strand replication. |
III | Myc tag to monitor expression in phage and bacteria or to add other things to test initial interaction with host. |
IV | Change size of protein to see effect of different channel sizes. |
V | Add more DNA binding sites to see if more DNA can be packaged into phage. |
VI | Myc tag to monitor expression in phage and bacteria or to add other things to test initial interaction with host. |
VII | Tag protein to monitor interaction with p5/DNA complex. |
VIII | Change size of protein to experiment with the size of coat. |
IX | Tag protein to monitor interaction with p5/DNA complex. |
X | Increase expression to see if more phage leave the host E. coli. |
XI | Change size of protein to see effect of different channel sizes. |
M13 Refactoring
Overlapping Areas | Original Sequence | Modified sequence |
---|---|---|
Gene 2 ORF and Gene 10 RBS | ||
Gene 10 ORF and Gene 5 RBS | ||
Gene 5 ORF and Gene 7 RBS | ||
Gene 5 ORF and Gene 9 RBS | ||
Gene 9 ORF and Gene 8 RBS |
The refactored genome is [Part BBa_M31332 | http://parts.mit.edu/registry/index.php/Part:BBa_M31332] on the Registry of Standard Biological Parts