User:Alji

Andrew Ji

About Me

• Year: 2009
• Major: Biological Engineering
• Minor: Economics
• Email: alji AT mit DOT edu

M13K07 Genome Re-engineering ideas:

Gene	Ideas
I	Change size of protein to see effect of different channel sizes.
II	Increase or decrease expression to optimize rate of + strand replication.
III	Myc tag to monitor expression in phage and bacteria or to add other things to test initial interaction with host.
IV	Change size of protein to see effect of different channel sizes.
V	Add more DNA binding sites to see if more DNA can be packaged into phage.
VI	Myc tag to monitor expression in phage and bacteria or to add other things to test initial interaction with host.
VII	Tag protein to monitor interaction with p5/DNA complex.
VIII	Change size of protein to experiment with the size of coat.
IX	Tag protein to monitor interaction with p5/DNA complex.
X	Increase expression to see if more phage leave the host E. coli.
XI	Change size of protein to see effect of different channel sizes.

M13 Refactoring

Overlapping Areas	Original Sequence	Modified sequence
Gene 2 ORF and Gene 10 RBS	At bp 478: gcatttgag	gcGtttgag
Gene 2 ORF and Gene 5 RBS	At bp 826: gcataaggt	gcGtaaggt
Gene 10 ORF and Gene 5 RBS	At bp 1286: gcataaggt	gcGtaaggt
Gene 5 ORF and Gene 7 RBS	At bp: 1609: gttccggct	gtCccggct
Gene 7 ORF and Gene 9 RBS	At bp: 1733: cgctggggg	cgAtggggg
Gene 9 ORF and Gene 8 RBS	At bp: 1869: aatggaaac	aaCggaaac

The refactored genome is Part BBa_M31332 on the Registry of Standard Biological Parts