User:Allison K. Alix/Notebook/CHEM-581/2013/02/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 100: Line 100:




'''Photos of hydrogels (MW= 22,000g/mol, 89,000 g/mol, 130,000 g/mol respectively)'''
'''Photos of hydrogels (MW= 22,000g/mol, 89,000 g/mol, 130,000 g/mol, and 146,000 g/mol respectively)'''


[[Image:2013-02-08 12.18.19.jpg|center|400px|]]
[[Image:2013-02-08 12.18.19.jpg|center|400px|]]
Line 110: Line 110:


[[Image:2013-02-08 12.21.31.jpg|center|400px|]]
[[Image:2013-02-08 12.21.31.jpg|center|400px|]]
[[Image:2013-02-08_12.25.11.jpg|center|400px|]]


==Observations==
==Observations==

Revision as of 13:22, 8 February 2013

Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

- Mimic Stomach Acid environment to verify that hydrogel core will dissolve without liposome shell

- Swelling Study of pure hydrogels in water

Procedures

1) Prepare several different low pH solutions (1.5 , 2.5, 3.5) using milli Q H2O and 1N Hydrochloric Acid

2) Place each MW in each pH solution as well as water. (4 tests for each MW, 12 in all)

3) Leave solutions for 5 days and monitor water absorbed by the hydrogels as well as whether or not they completely dissolve in the "stomach acid"

Data

Masses of hydrogels used for stomach acid/water swelling studies

146,000 g/mol

pH mass (g)
1.5 0.18
2.5 0.16
3.5 0.18
water 0.22






130,000 g/mol

pH mass (g)
1.5 0.54
2.5 0.56
3.5 0.43
water 0.66






89,000 g/mol

pH mass (g)
1.5 0.58
2.5 0.59
3.5 0.60
water 0.55






Photos of hydrogels (MW= 22,000g/mol, 89,000 g/mol, 130,000 g/mol, and 146,000 g/mol respectively)




Observations

-22,000 g/mol PVOH did not form a hydrogel. After three freeze thaw cycles (24 hours @ -20°C, 24 hours at room temperature) the sample was still very much a liquid. This being said, we have concluded this molecular weight is not suitable for our drug delivery system.

-nanospheres that were prepared on Wednesday may be too small for our system. We are looking to alter the procedure slightly so we can make spheres slight bigger (micro or even milli scale)



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Allison_K._Alix/Notebook/CHEM-581/2013/02/08&oldid=674768"