User:Allison K. Alix/Notebook/CHEM-581/2013/02/11
|Line 33:||Line 33:|
|Preparing Microgels Part II|| Main project page|
Previous entry Next entry
The nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale)
1) Add 0.5g of PVOH (MW = 89,000-98,000 g/mol) to 6mL phosphate buffer solution
2) Heat at 100°C for ~15 minutes
3) Pour into 50mL mineral oil and homogenize on the lower setting (rpm unknown- unlisted) for 1 min
4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles.
-Size of hydrogels is still unknown, but we are hoping that using more PVOH/phosphate buffer solution and less mineral oil as well as homogenization at a lower speed will allow for the formation of larger hydrogels.