User:Allison K. Alix/Notebook/CHEM-581/2013/02/11

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Objective

  • Prepare smaller hydrogels using a similar procedure to the one used on 2/06/13

Description

The nanogels prepared on 2/06/13 may be too small for our system. This being said, we are looking to devise another procedure using w/o emulsion that will make slightly larger hydrogels (~microscale)

Procedures

1) Add 0.5g of PVOH (MW = 89,000-98,000 g/mol) to 6mL phosphate buffer solution

2) Heat at 100°C for ~15 minutes

3) Pour into 50mL mineral oil and homogenize on the lower setting (rpm unknown- unlisted) for 1 min

4) Place in freezer at -20°C for 20 hours. Thaw for 4 hours. Repeat this cycle for a total of 3 cycles.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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