User:Allison K. Alix/Notebook/CHEM-581/2013/02/20: Difference between revisions
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* Freeze using Liquid Nitrogen before placing in freezer | * Freeze using Liquid Nitrogen before placing in freezer | ||
== | ==Procedures== | ||
Make the following samples: | Make the following samples: | ||
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3) 0.14g PVOH, 3mL buffer, 50 mL oil | 3) 0.14g PVOH, 3mL buffer, 50 mL oil | ||
Set 1: | |||
Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C | |||
Set 2: | |||
Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C | |||
Set 3: | |||
Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing | |||
==Data== | ==Data== | ||
Revision as of 07:19, 20 February 2013
 Microgel Prep III
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Objective
ProceduresMake the following samples: 1) 0.5g PVOH, 4 mL buffer, 50 mL oil 2) 0.25g PVOH, 3 mL buffer, 50 mL oil 3) 0.14g PVOH, 3mL buffer, 50 mL oil Set 1: Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C Set 2: Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C Set 3: Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing DataNotes | |
