User:Allison K. Alix/Notebook/CHEM-581/2013/02/20

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objective)
(Procedures)
Line 28: Line 28:
Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C
 +
 +
*done for all samples
Set 2:
Set 2:
Line 38: Line 40:
Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing
Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing
 +
 +
*done for all samples
 +
 +
Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing
==Data==
==Data==

Revision as of 11:52, 20 February 2013

Microgel Prep III Main project page
Previous entry      Next entry


Objective

  • Use Safflower Oil (higher freezing point than mineral oil)
  • Freeze using Liquid Nitrogen before placing in freezer

Procedures

Make the following samples:

1) 0.5g PVOH, 4 mL buffer, 50 mL oil

2) 0.25g PVOH, 3 mL buffer, 50 mL oil

3) 0.14g PVOH, 3mL buffer, 50 mL oil

Set 1:

Use Safflower Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • done for all samples

Set 2:

Use Mineral Oil, blend for ~2-3 min at highest speed, pour into large petri dishes, and place in freezer at -20°C

  • only done for 0.25g sample

Set 3:

Use Safflower oil, blend for 2-3 min and freeze with liquid nitrogen prior to freezing

  • done for all samples

Repeat a 3 Freeze-Thaw Cycles of 20 hours freezing and 4 hours thawing

Data

Notes


Personal tools